空间位阻对溶解态甲基菲生物降解的影响.PDFVIP

中国环境科学 2017,37(4) ：1375~1379 China Environmental Science 空间位阻对溶解态甲基菲生物降解的影响 杜 兰 1,张振轩 1,朱亚先 2,张 勇 1* (1.厦门大学环境与生态学院,近海海洋环境科学国家重点实验室,福建 厦 门 361102 ；2.厦门大学化学化工学院化学系,福建 厦门 361005) 摘要： 以菲(Phe)和甲基菲(MP:1-MP 、3-MP 、4-MP 、9-MP)为研究对象,运用同步荧光法研究微生物 Novosphingobium pentaromativorans US6-1 对 5 种溶解态多环芳烃(PAHs)的降解特性.结果显示:MP 在 8×10-7,1.2×10-6mol/L2 种浓度下,其生物降解过程符合零级动力学,降解 速率均为:4-MP9-MP1-MP3-MP;5 种 PAHs 的最大生物降解速率同理论最大跨膜通量存在良好的线性关系;NaN 对 Phe 降解速率影响 3 较小,但抑制 MP 降解且抑制程度与取代基位置有关. 关键词： 甲基菲；同系物；生物降解；跨膜过程 中图分类号：X127 文献标识码：A 文章编号：1000-6923(2017)04-1375-05 1 1 Effect of steric hindrance on the biodegradation of dissolved methyl-phenanthrene. DU Lan , ZHANG Zhen-xuan , ZHU Ya-xian2, ZHANG Yong1* (1.State Key Laboratory of Marine Environmental Science of China, College of Environment and Ecology, Xiamen University, Xiamen 361102, China；2.Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China). China Environmental Science, 2017,37(4) ：1375~1379 Abstract ：The biodegradation of dissolved phenanthrene (Phe) and methyl-phenanthrene (MP), including 1-MP, 3-MP, 4-MP and 9-MP by the bacterium Novosphingobium pentaromativorans US6-1was individually studied using synchronous fluorescence spectrometry. The biodegradation of MP was adequately described by zero-order kinetics at two concentrations, i.e., 8×10-7mol/L and 1.2×10-6mol/L. The rates of biodegradation were generally in the following order: 4-MP9-MP1-MP3-MP. There was a good linear relationship between the measured maximum biodegradation rates and the theoretical maximum