姜黄素结合有氧运动对中老年中大鼠骨骼肌aktmtor信号通路的影响.docxVIP

ABSTRACTObject ABSTRACT Object i ve：By detecting the rat soleus muscle IGF一1，change Pi3k／Akt／mTOR protein expression of its content to cell apoptosis，explore curcumin，constant load exercise，incremental Exercise on Pi3k／Akt／mTOR signaling pathway．And comparing curcumin，constant load exercise，incremental load exercise， combined with constant load exercise curcumin， curcumin combined rat skeletal muscle aging effect of exercise on increasing load delay． Methods：The same batch of 18 healthy male SD rats were 60 months of age(555．61±57．449)，SPF(Specific pathogen Free，SPF) level of animals，housed separately．Adaptive feeding for three days，were randomly divided into six groups，were randomly divided into control group(Control group，BC，n=lO)，curcumin (Curcumin group，QC，n=lO)，constant load exercise group (Constant load exercise group，CE，n=10)，increasing load exercise group(incremental Exercise group，IE，n=lO)，a constant load exercise+curcumin group(constant load exercise curcumin group，CC，n=10)，incremental Exercise+curcumin group(Incremental Exercise+curcumin group，IC，n=lO)．2 points each to cages，five per cage．Exercise group rats using treadmi ll exerci se，the establ i shment of a constant load and strength 19m／min X 60min，increasing load exercise intensity Referring to Table卜2．Slope of 0(equivalent to Exercise Intensity 70％V02max)，five days a week for training， two days of rest，training a total of 8 weeks．After the end III 万方数据 of of the last 20 hours of training to take blood，soleus muscle was measured IGF一1 content using E1 issa method，using Western Blotting Detection Akt，Pi3k，mTOR protein expression，and the situation of skeletal muscle cell apoptosis by TUNEL assay． Resu l ts：(1)Muscle apoptosi sin rat soleus smal lest index is constant load exercise group，followed by the curcumin group， apoptotiC index 1S the largest group lncreaslng load．Compared with curcumin group and constant load group，there was a significant difference P0．01：compared with curcumin increas