基因重组人生长激素对肝癌细胞系生长的影响-外科学专业论文.docxVIP

基因重组人生长激素对肝癌细胞系生长的影响中文摘要 基因重组人生长激素对肝癌细胞系生长的影响 中文摘要 目的： 研究基因重组人生长激素(rhGH)作用人肝癌细胞系后细胞周期动 力学的改变，了解其对人肝癌细胞系生长的影响。为指导临床肝癌病人围术期能 否使用rhGH纠正病人负氮平衡提供理论依据 方法：不同浓度rhGH(0、2、5、25、50、150、500肛g／L)作用于人肝癌细 胞系HepG2，以细胞计数、MTT法测定rhGH对肝癌生长及增殖的影响，采用流 式细胞仪检测作用24，48，72h后的细胞周期动力学，分析其增殖指数、s期细胞 百分比及细胞凋【=：=率的变化。 结果：统计分析显示rhGH作用后的HepG2细胞周期动力学改变与作用时间 无显著相关：与对照组比较，在生理浓度(25垤／L)rhGH作用下，HepG2细胞增 殖明显(PO．05)，PI及s％亦显著上升(P0．05)；在超生理浓度(500肛g／L)rhGH 作用下， PI及S％无显著改变(P0．05)，但细胞增殖却明显受抑制(P0．05)； 另2、5、25、50、150、500¨g／L rhGH均诱导人肝癌细胞凋亡，且呈浓度一效应 关系。 结论：1．在体外培养体系中， rhGH在生理浓度或低于生理浓度下促进肝 癌HepG2细胞的增殖，在高于生理浓度下抑制肝癌HcpG2细胞的增殖。2间接 证明肝癌HepG2细胞有生长激素受体的存在，其GH．GHR．IGFs信息通路是完 整的并且功能没有缺陷。3．rhGH在体外培养体系中促进肝癌HepG2细胞的凋亡， 并呈现出剂量一效应关系。 关键词人生长激素肝癌细胞细胞周期动力学凋亡增殖 Study Study on the effect of recombinant human growth hormone on the growth of human hepa-toma cell line Abstract objectives：To investigate the effect of recombinant human growth hormone (rhGH)on the growth of a human hepatoma cell line and to provide theoretic evidence on guiding the usage of the rhGH to correct the negative nitrogen balance state during the perioperative period in the treatment ofliver carcinoma patients． Methods：Various concentrations of rhGH(0,2，5，25，50，150，500刚L)were added to the cell lines(HepG2)cultured in DMEM medium for 24，48，72hr．Thereafter the cell growth and proliferation were measured by cell counting and M丌assay． the cell cycle kinetics was measured by flow cytometry and the proliferation index(PI)，the percentage of apoptosis and S phase(S％)were analyzed． Results：Statistics analysis showed that the effect of rhGH on cell cycle kinetics were not concerned丽th time but concentration，Compared with the control， HepG2 cell line proliferated significantly．PI and S％also increased significantly at physiological concentration of rhGH(25pg／L)0o．05)but PI and S％showed no difference at supraphysiological concentration of rhGH(50099／L)(p0．05)，while HepG2 cell line proliferation was inhibited effectively by rhGH at supraphysiological concentration of(50009／L)