基因工程酵母发酵木糖生产酒精的研究-生物化工专业论文.docxVIP

浙江大学硕士学位论文 浙江大学硕士学位论文 摘要 等四种方法进行脱毒处理。结果表明，真空浓缩汽提可脱去大部分乙酸和糠醛， 再结合石灰中和除去半纤维素水解液中的S04}，则是一种简单有效的半纤维素 水解液脱毒工艺。利用基因工程酵母固定化细胞对脱毒后的半纤维素水解液进行 酒精发酵，71．8 edL木糖发酵72 h后残留木糖浓度为2．1 g／L，木糖利用率为 97．1％，生成31．1 g／L酒精。 利用基因工程酵母最cerevisiae ZU—10固定化细胞发酵玉米秸秆酶解液中的 葡萄糖和木糖，酶解液先经氢氧化钠预处理，24 h内66．9 g／L的葡萄糖和32．1 g／L 的木糖发酵生成40．7 g／L的酒精。同S cerevisiae ZU一10游离细胞相比，固定化 细胞发酵缩短了发酵周期，大大提高了生产效率。利用固定化细胞重复12批发 酵玉米秸秆酶解液，葡萄糖利用率均为100％，木糖利用率均在90％以上，平均 酒精浓度为40．4g／L，对葡萄糖和木糖的平均酒精得率为O．41，结果表明固定化 细胞可以持续、高效、稳定地发酵玉米秸秆酶解液生成酒精。 关键词：木糖，酒精发酵，基因工程酵母，固定化细胞，玉米秸秆，半纤维素水 解液，酶解液 II 浙江大学硕士学位论文 浙江大学硕士学位论文 ABSTRACT ABSTRACT Bioconversion of xylose to ethanol is an important process in ethanol production from plant lignocellulosic materials．Sacchromyces cerevisiae has traditionally been used for ethanol fermentation，but it Can’t utilize xylose，Several yeasts，such as Pichia sapitis and Candida shehatae，Call be used for the ethanol fermentation from xylose．However,their ethanol tolerance is low and semi-oxygen is required for optimal ethanol productivity．In this thesis，a recombinant yeast，Sacchromyces cerevisiae ZU-10，is used for the ethanol fermentation from xylose by free and immobilized cells，and then the ethanol production from lignocellulosic materials were investigated． The suitble xylose concentration，initial pH value and tempetature of xylose fermentation were 80 g／L，5．5 and 30℃respectively,and 29．3 g／L ethanol was obtained within 96 h．However,ethanol fermentation by-S：cerevisiae ZU-i0 would be impacted obviously when there was higher than 0．25 g／L acetic acid or higher than 0．08 g／L furfural in xylose substrate． Addition of glucose favored xylose fermentation．85．7％xylose was utilized within 36 h when adding 40 g／L glucose to culture containing 30 g／L xylose．This research was valuable for industrial application ofX cerevisiae ZU-10． The recombinant yeast of．S．cerevisia