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igtcr基因信重排原理及多样性
* * * * * * * * * * * * * Chapter4 The generation of lymphocyte antigen receptors Primary immunoglobulin gene rearrangement Antibody repertoire : 1011 in human - The total number of antibody specificities available to an individual Ig gene rearrangement in antibody producing cells - Southern blot analysis non lymphoid cell : V,C in the distant fragments mature B cell : V,C in the same fragments ? somatic recombination Figure 4.1 - V gene rearrangement Figure 4.2 V region of light chain : V gene segment (VL protein) (VL gene) J gene segment (JL gene) V region of heavy chain : V gene segment (VH protein) (VH gene) D gene segment (DH gene) J gene segment (JH gene) - The numbers of functional gene segments for the V region of human heavy and light chains ′ pseudogenes ′ : not functional protein Figure 4.3 The germline organization of the Ig genes gene family Figure 4.4 Rearrangement of V,D,J gene segments - DNA rearrangements are guided by conserved noncoding DNA sequence Figure 4.5 (recombination signal sequence, RSS) ? heptamer : contiguous with the coding sequence ? space : 12 or 23 nucleotides long ? nonamer : second conserved sequence - coding sequence-heptamer-space-nonamer (12/23) - 12/23 rule - exception : D-D joining (fusion) in human ≈ 5% - two mode of rearrangement Figure 4.6 ? looping out and deletion : more
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