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29(4)561-568 Chinese Journal of Biological Control 2013 11
*
210023
16S rDNA
G9R15 J13
Pseudomonas fluorescens G9 R15 J133040
dG9 R15 15 d J13
915 1520 dG9
5 −1 5
9.7310 cfu·g 100%6 dR15 6.3010
cfu·g−1 100% 14 d
S476 1005-9261201304-0561-08
Colonization Dynamics of Endophytic Bacteria in Capsicum annuum and Their Control
Effects on Pepper Phytophthora Blight
*
FU Siya, CHEN Shuanglin, YAN Shuzhen
(Collage of Life Science, Nanjing Normal University, Nanjing 210023, China)
Abstract: In order to clarify the relationships between colonization dynamics of endophytic bacteria and the control
effects on pepper Phytophthora blight, endophytic bacterial strains were screened by dual culturing against
Phytophthora capsici and control effects were measured on the potted seedlings. The endophytic bacteria were
identified based on their morphological characteristics, biochemical characteristics and 16S rDNA sequence
BLAST. Colonization dynamics of endophytic bacteria in seedlings of Capsicum annuum were also conducted by
rifampicin labelling and their relationships with control effects were analyzed. The results showed that three
endophytic bacterial strains G9, R15 and J13 had obvious control effects against P. capsici and they were identified
as Pseudomonas fluorescens . The strains G9 and R15 could colonize in roots of C. annuum, and J13 could colonize
in roots, stems and leaves
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