基因芯片表达数据的标准化策略研究-卫生统计学专业论文.docxVIP

第三军医大学硕士学位论文 第三军医大学硕士学位论文 39 39 英文缩写词表 (按英文字母排序) 英文缩写 英文全称 中文参考译文 ANOVA analysis of variance 方差分析 CSN control spots normalization 参照点标准化 DNA deoxyribonucleotide acid 脱氧核糖核酸 GEM gene expression matrix 基因表达矩阵 GEC gene expression chip 基因表达型芯片 LMN locally mean normalization 局部均值化标准化 LOWESS locally weighted scatter-plot smoothing 局部加权散点图滤波 LWLRN locally weighted linear regression normalization 局部加权线性回归标准化 Mean mean 均值或平均值 Median median 中值或中位数 MI mutual information 共享信息 mRNA message ribonucleotide acid 信使核糖核酸 PCA principal component analysis 主成分分析 RNA ribonucleotide acid 核糖核酸 SAM significance analysis of microarray 芯片显著性分析 SOM self-organize map 自组织图 SVM support vector machines 支持向量器 S signal 信号 SV systematic variation 系统变异 TIN total intensity normalization 总强度标准化 论文中下标字符意义说明 i 表示芯片样本中的某个杂交点 通常该点对应某一个基因 i=1,2, ,N j 表示微阵列网格中的某个子集网格(或块) j=1,2, , M k 表示基因芯片实验中某个样本(如实验样本 对照样本 通常有 k=1,2) l 表示重复的试验次数 l=1,2, ,R Research of Normalization Strategy of expression data for Gene Chip Abstract Motivation: With the rapid development of molecular biological and computational technologies, the implement of HGP and achievement of HGD show that modern life science research has entered the post-genomic era, researchers focus on from structure genome science to function genome science. The DNA microarray is capable of profiling the expression levels of many genes simultaneously, and is a promising technology for the research of gene interactions. And how to exactly and reasonably analyze the large of data that result from the microarray experiments has become a problem that researchers are eager to resolve, and important research content of bioinformation and main method. Now there have been quite a few methods including sophisticated mathematical models that are used to analyze image and data of gene chip, especially normalization of data analysis earlier, which will be perfected and innovated further. Normalization is the process of removing some sources of variation which affect the