小儿造血与血液特点.ppt

Embryo development and tissue (cell) commitment Comparisons of hematopoietic locations between preschool children and adults Dynamic changes of RBC during infancy Low limits of Hb in different age groups Newborns 145 g/L 1～4m 90 g/L 4～6m 100 g/L 6m～6y 110 g/L 6y～14y 120 g/L 4% of increase per 1000 meter above sea level. Molecular structure of hemoglobin Types and proportions of hemoglobin during embryo, fetus and infancy stages Different proportions of Hb during infancy and early young children Blood picture in children (4) Platelets: normal 100 ~ 300 x109/L Total blood volume: Newborn: 10% of body weight Childhood: 8 ~ 10% of BW Adults: 6 ~ 8% of BW Important nutritional components for erythropoiesis Iron Vitamin 12 Folic acid Features of iron metabolism in children Iron store: Metabolism of Vit B12 and Folic acid Folic acid: food: rich in green vegetable, lack in sheep milk ; Absorptive site: at upper portion of jejunum; Clinical relevant cytokines or hormones associated with hematopoiesis EPO G-CSF GM-CSF IL-11 TPO Clinical Application of CD34+ cells CD34 as marker of HSC or HPC for quantitation Clinical Application of CD34+ cells Thank you for attention ! Hyperfragmented neutrophil * Childhood hematopoiesis and hematological features Hematopoietic Stem Cells (HSC), CD34 is the marker of HSCs. 5 um All the daughter blood cells are derived from the HSCs. Early development of the fertilized egg. Development of hematopoietic stem cells PLURIPOTENT STEM CELL CD34+CD38- HPP-CFU, LTC-IC,CFU-BL MYELOID STEM CELL CD34+CD38- CD33+CD13+ LYMPHOID STEM CELL TdT+CD34+ BFU-MK CD34+ CD61+/- BFU-E CD71+ CD36+/- CFU-GM CD34+CD33+ CFU-M CFU-G CD19+ T PROGENITOR CD7+ TOTIPOTENT STEM CELL T4 T8 CFU-E CD71+ CD36+ CFU-MK CD61+ S