白腐菌糙皮侧尔漆酶性质及其对蒽醌染料脱色性能的研究.PDF

24 1 Vol.24 No. 1 2004 3 Chemistry and Industry of Forest Products Mar. 2004 1, 2 1 1 1 1 侯红漫 , 周集体 , 王 竟 , 杜翠红, 严 滨 ( 1. 大连理工大学 环境科学与工程学院, 辽宁大连 116011; 2. 大连轻工业学院生物与食品工程学院, 辽宁大连116034) HOU H M : ( Pleurotus ostreatus strain 3.42) , ( LiP) (MnP) 0. 5 mmol 2, 2- - (3- - 6- ) (AB S) 0. 1 2+ mmol Cu , 1 000 800 U/ mL, pH 3.5, pH 4.0~ 6.0 ; 65 , (pH 5.0) , 50 , ; - - KN- R(RBBR) (30 U/ mL) , 12h 70% ; AB S(5 mol/ L) , : ; ; : Q554 :A : 0253- 2417( 2004) 01- 0048- 05 CHARAC ERIS ICS OF LACCASE FROM WHI E-RO FUNGUS PLEUROTUS OSTREATUS S RAIN 3. 42 AND I S DECOLORIZA ION EFFEC ON AN HRAQUINONE DYE 1,2 1 1 1 1 HOU Hong-man , ZHOU Ji-ti , WANG Jing , DU Cu-i hong , YAN Bin ( 1. College of Environmental Science and Engineering, Dalian University of Tec nology, Dalian 116011, C ina; 2. Department of Biologic and Food Engineering, Dalian Institute of Lig t Industry, Dalian 116034, C ina) Abstract: White-rot fungus Pleurotus ostreatus strain 3.42 produces extracellular laccase,but no lignin peroxidase(LiP) and manganese peroxidase(MnP) are detected in the supernatant when grown in liquid culture.When [2, 2- azino-bis( 3-ethyl- benzthiazoline-6-sulfonic acid,AB S) ] (0. 5 mmol/ L) and Cu2+ (0. 1 mmol/ L) were used as inducer of laccase production, high activity level up to 1 000 and 800 U/ mL was obtained respectively. Compared with