地塞米松对ANIT诱导的胆汁淤积大鼠肝胆系统AQP1表达的影响-消化内科学专业论文.docxVIP

- - PAGE 2 - 肿胀、坏死及其脱落碎屑阻塞胆管腔,胆管增生,肝小叶轮廓不清,汇管区大量炎细 胞浸润,肝细胞浊肿、空泡,嗜酸性变,坏死明显。BCD 组又以 B 组重， D 组 C 组病 理改变依次有所减轻,但差别不大。 6.免疫组化切片光密度值(OD Value)：A 组胞膜可见棕黄色颗粒。B 组表达明 显为少，少见于胞膜。CD 两组可见胞膜胆管侧有表达。A 组免疫组化光密度值要 明显高于 B 组(P0.01)。BCD 三组中，C 组高于 BD 组(P0.01)，D 组略高于 B 组. 差别不明显(P0.05). 7.OD 值与 BC 两变量行 Spearman 相关分析：分解率为 0.885(P0.01)，提示 两者密切相关。 结论：ANIT 一次性灌服可成功诱导大鼠胆汁淤积。DEM 干预组 AQP1 表达增多， 胆汁分泌量增加，AQP1 的表达与胆汁量密切相关。胆红素指标下降，DEM 有减黄 作用。提示临床对因化学性肝损引起的急性黄疸病例可早期用激素制剂干预。 关键词：水通道蛋白 1，胆汁淤积，地塞米松，肝胆系统，α-萘异硫氰酸 Influence of dexamethasone on AQP1 expression in hepatobiliary system of rats with ANIT-induced cholestasis Abstract Objective：This study was to induce rat models of cholestasis by α-isothiocyanate (ANIT) and dexamethasone treatment was on . Changes of rat liver structure 、liver function and the capacity of bile were observed, using immunohistochemical methods to detect the aquaporin1 expression and their expression laws. To study the influence of dexamethasone on hepatocyte function of bile secretion and explore its impact mechanism. Methods：32 healthy adult male Wistar rats were randomly divided into 4 groups. The group treated with only peanut oil is the group A.B group is the group treated with only ANIT eanut oil.C group is the group treated with ANIT eanut oil and injected with dexamethasonea at the same time.D group is the group treated with ANIT eanut oil and injected with dexamethasonea after 24 hours. All rats were killed after 48 hours to collect samples.A single oral dose of 150 mg/kg ANIT dissolved in peanut oil was given to rats by gavage(10g/l) to induce liver injury. Intraperitoneal injection was given with dexamethasone (10 mg/kg) compatibl 2 ml normal saline. The common bile duct of all rats were intubated to collect bile in one hour , portal blood were send to test liver function with automatic biochemical analyzer. Liver specimens stained HE were on light microscopic observation. Immunohistochemical methods detected aquaporin1 expression. Three sections of each rat were image