水稻矮秆脆性突变体dbc1的鉴定与基因定位-中国烟草学报.PDF

作物学报 ACTA AGRONOMICA SINICA 2013, 39(4): 626631 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2013.00626 水稻矮秆脆性突变体dbc1 的鉴定与基因定位 桑贤春 杜 川 王晓雯 杨正林 凌英华 赵芳明 李云峰 何光华* / / , 400716 : EMS 10 , dbc1, , , , 58.93 cm, , dn , , , , dbc1 , , , 1 , 886 1A/dbc1 F2 , DBC1 2 SSR RM13943 RM13952 , 197 kb, 52 dbc1 : ; ; ; ; Identification and Gene Mapping of Dwarf and Brittle Culm Mutant dbc1 in Oryza sativa SANG Xian-Chun, DU Chuan, WANG Xiao-Wen, YANG Zheng-Lin, LING Ying-Hua, ZHAO Fang-Ming, * LI Yun-Feng, and HE Guang-Hua Rice Research Institute of Southwest University / Chongqing Key Laboratroy of Application and Safety Control of Genetically Modified Crops / Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 400716, China Abstract: One dwarf and brittle culm mutant dbc1 was identified from the progeny of restorer line Jinhui10 which seeds were treated by ethyl methanesulfonate (EMS). From the seedling stage to the mature stage, dbc1 showed obvious characteristics of dwarfism and frangibility. In detail, the mutant shortened each internode with a plant height of 58.93 cm, belonging to the dn type of dwarfism mutation. Treated by GA3, the dbc1 showed more insensitive compared with its original parent Jinhui10. One thou- sand grain weight, seed-setting rate, and tiller numbers did not change while panicle length, grain number p