依达拉奉对小鼠蛛网膜下腔出血早期erk12调控海马神经元调亡义及vegf介导脑动脉内皮细胞凋亡的影响.docx

附图附表 附图附表 00 00 24 讨论································································“”一“”一”27 小结············································· ·“”一”“一”一一”” ·29 参考文献 29 第二部分 小鼠蛛网膜下腔出血早期ERKl／2、Bax、caspase．3调控海马 神经元凋亡及依达拉奉的治疗作用 前言刖舌·······························································”一··”””32一一一”一“”一“一一“”一”“”““一””””””””一””””””””。jZ 材料与方法 33 结果····-···- ···················· ····一·····”··················“一··”一”42 附图附表 46 讨论·································“····一··························”···”一”55 小结··················································-············ ···· ”58 参考文献 58 第三部分 小鼠蛛网膜下腔出血早期血管痉挛与VEGF介导的脑动脉内 皮细胞凋亡的关系及依达拉奉的治疗作用 前言”一”“一““一”“一””一””””．．一““．．””一““一”“一””一”一一”0—2刖舌”一”“”““一”“一””一””””””“““””““““”“一””一”“一” 材料与方法 62 结果············ ························ ································．．”70 附图附表 73 讨论··············································································78 讨论··············································································78 小结··················IbQIb OIIbl···-·············· ···························79 参考文献 79 结论·····················-························································82 综述蛛网膜下腔出血早期脑损伤的实验研究进展 83 致谢··············································································1 04 个人简历 105 中文摘要依达拉奉对小鼠蛛网膜下腔出血早期ERKl／2调控海马神经元 中文摘要 依达拉奉对小鼠蛛网膜下腔出血早期ERKl／2调控海马神经元 凋亡及VEGF介导脑动脉内皮细胞凋亡的影响 摘 要 第一部分 小鼠蛛网膜下腔出血模型制备及评价 目的： 制作小鼠血管内插线致蛛网膜下腔出血模型，并对该模型的 死亡率、神经学改变及蛛网膜下腔出血的程度进行评价。 方法： 1实验动物及分组体重289．329雄性昆明小鼠30只，随机分为假手术 组6只、模型组24只。 2模型制备采用血管内插线法建立小鼠蛛网膜下腔出血模型。手术显微 镜下行颈部正中切口，依次分离右侧颈总动脉、颈外动脉和颈内动脉。颈 外动脉结扎并剪断，与颈内动脉拉成直线，将头