一种微孔板生物毒性快速检测技术义的建立-环境工程专业毕业论文.docxVIP

ABSTRACTThis ABSTRACT This paper described a multi—species 1 8一h microbial assay,TOXPlate，which developed here for acute toxicity assessment of pure chemicals and environmental samples．A tetrazolium dye，2,3，5一triphenyltetrazolium chloride(TTC)，WaS used as an indicator to test bacterial growth inhibition in each well of microplate．Measurement of the growth of the organisms exposed to a range of concentrations of tested chemicals is determined by the reduction of TTC After being tested by several chemicals，eleven different indigenous strains WaS selected over dozens of candidates which were all from Environmental Culture Collection．The s订ains were tested for biodiversity,susceptibility,reproducibility and stability during reaction witll the growth indicator and response to the tested chemicals．These ten prokaryotic species and one eukaryote(yeast)，which are phylogenetically and biologically diverse from each other,constitute the biological indicators of the TOXPlate toxicity assessment：Comamonas testosterone, Brevibacterium epidermidis,Staphylococcus sciurf，Diaphorobacter nitroreducens, Brevundimonas diminuta,Pseudomonas Sp．．Ochrobactrum anthropi,Citrobacter freundi‘Pantoea agglomerans,Enterococcus casseliflavus,Saccharomyces Cg，PVISZd已． The optimum concentration of indicator as well as the inoculums of every individual strains of exact density Was determined to improve the test performance with a higher sensitivity．An assay using different concentrations of TTC showed good responses for concentrations ranging from 0．005％to O．01％(w／v)．It was also observed that strains showed different sensitivity to TTC，which would defmitely affect their colorimetric reaction．0．0 1％Was used as the testing concentration of TTC in TOXPlate． Sensitivity of TOXPlate was tested with the exposure to several pure chemicals and MTC(Microbial Toxic Concentration)value Was calculated．With a comparison between TOXPlate and some other acute toxicity test，it is concluded that the test we pres