马氏珠母贝磺基转移酶基因的克隆及功能分析-水产养殖专业论文.docxVIP

优秀毕业论文 精品参考文献资料 板块边缘出现明显的C 板块边缘出现明显的C轴生长。说明PmCHSTl la可能通过影响外套膜外液中 糖胺聚糖含量的变化，参与珍珠层的形成。 PmCHSTllb全长1 540 bp，编码392个氨基酸，含有磺基转移酶 (Sulfotransfer 2 domain)结构域，具有跨膜结构和信号肽，定位于高尔基体上。 组织表达差异分析发现，PmCHSTl lb在马氏珠母贝中央膜显著高表达(p0．05)。 RNA干扰后PmCHSTl lb在外套膜中央膜的表达量显著下调(p0．05)；外套膜 外液中糖胺聚糖浓度显著降低(pO．05)；贝壳珍珠层表面凹凸不平，结晶板块 边界模糊，表面粗糙。说明PmCHSTl lb可能通过影响外套膜外液中糖胺聚糖 含量的变化，参与珍珠层的形成。 3．马氏珠母贝磺基转移酶基因PmCHST9俾fucata martensii Carbohydrate sulfotransferase 9)的克隆与组织分布分析。PmCHST9序列全长l 388 bp，开放 阅读框(ORY)为1 074 bp，编码357个氨基酸；氨基酸序列分析得出PmCHST9 具有典型的磺基转移酶结构域(Sulfotransfer．2 domain)和一个跨膜结构域；多 序列比对结果显示PmCHST9与其它物种的CHST9同源性较低；荧光定量PCR 检测结果表明PmCHST9在鳃中显著高表达(p0．05)。因此推测PmCHST9可 能参与马氏珠母贝的免疫调节。 关键词：马氏珠母贝，磺基转移酶，糖胺聚糖，珍珠质，RNAi Ⅱ 万方数据 Cloning Cloning and Function Analysis of Sulfotransferase Genes in Pinctadafucata martensii Abstract Sulfotransferase plays a vital role in catalyzing the transfer of sulfonic acid groups in the processes of glycosaminoglycan synthesis，and glycosaminoglycans consist of many copies of negatively charged sulfonic acid groups participate in the nucleation process of biomineralization．In this study，we have cloned the full length sequences of five sulfotransferase genes using the RACE technology，studied their expression patterns by real—time PCR．The potential functions of these five sulfotransferases in nacre formation explored by RNAi technology，and studied the effect of sulfotransferases in the synthesis of glycosaminoglycans at the same time． The results are as follows： 1．Two keratan sulfate sulfotransferase PmCHST 1 were cloned，they were named PmCHST l a(P f“cata martensii Carbohydrate sulfotransferase l a)and PmCHSTlb fP．j励cata martensii Carbohydrate sulfotransferase lb)． PmCHSTla：The full length was 1 385bp，encoded for a protein ofa 366 amino acids．PmCHSTl a carried with a Sulfotransferase domain(Sulfotransfer-3 domain)． a signal peptide and transmembrane domain which made the protein located in the Golgi apparatus．Real-time PCR results showed that PmCHST l a was significantly highly expre