马氏珠母贝植核育珠移植耐受免疫相关基因与蛋白质鉴定及分析-海洋生物学专业论文.docxVIP

优秀毕业论文 精品参考文献资料 10天与植核前相比，有 10天与植核前相比，有37个上调蛋白、60个下调蛋白；20天与10天相比，得到29 个差异蛋白，其中21个上调，8个下调；30天与20天相比，有12个上调差异蛋白、 9个下调差异蛋白。获得的免疫相关的差异蛋白质有原钙黏蛋白(Protocadherin)、血 纤维蛋白溶酶原(Plasminogen)、细胞凋亡蛋白酶(caspase)、干扰素诱导蛋白、白 介素17受体、肿瘤坏死因子、肿瘤坏死因子相关因子、热休克蛋白70、TRIM家族 蛋白、TNF—a诱导蛋白、Notch家族蛋白、抗菌L．氨基酸氧化酶、E3泛素连接酶、 整合素、死亡相关蛋白激酶、肽聚糖识别蛋白等。 本研究分别从转录组和蛋白质组两个方面研究了马氏珠母贝植核育珠后不同时 期血淋巴中与免疫相关的差异表达基因和蛋白质，不仅丰富了马氏珠母贝的eDNA 和蛋白质数据，而且为进一步了解育珠过程中机体免疫的变化规律及揭示马氏珠母贝 耐受移植的分子机制奠定基础，进而更加深入探究无脊椎动物非特异性免疫相关机 制。 关键词：马氏珠母贝，转录组测序，蛋白质组，iTRAQ Il 万方数据 J一 J一 ■● Identification and Analysis of Immune Tolerance Related benes and rrotelns in ctadafucata martensii after the Nucleus．．insertion Abstract Pinctada fucata martensii is the major marine pearl oyster cultured to produce artificial pearl．The core part of marine pearl culture is the necleus-inserting operation．The operation is to insert pearl necleus and mantle pieces from the donor pearl oyster into the visceral mass of the recipent for nucleation．The issues and mantle pieces form pearl sac together．The pearl sac cells secrete nacre which deposited on the necleus forming the pearl． The mantle pieces inserted could cause immune rejection ofthe pearl oyster for nucleating， which may cause the discharge of necleus and even the death of pearl oyster．Therefore， researchers and culturists have taken various measures in an attempt to ease the immune rejection after necleus—inserting opration for a long time．But SO far these problems have not been solved，for which the fundamental reason is that the stressed immune response key factors in the process of necleus—inserting opration are unknown． In this study,haemolymph transcriptome libraries from different stages of P fucata martensiiwere constructed，sequenced and assembled by RNA-seq approachand analyzed． The differential expression of functional genes and key signal transduction pathways were analyzed and screened in order to identify the immune—related genes．Furthermore，the immune—related proteins were screened and identified by