基于绿色合成纳米材料的电化学发光生物传感器的构建及其应用-物理化学专业论文.docxVIP

优秀毕业论文 精品参考文献资料 Abstract Abstract E1ectrochemiluminescence(ECL)is a process of converting electrochemlcal the sunace of energy into luminous energy via an electron transfer reaction at electrode．Recent years have witnessed the great development of ECL sensor as a platfom for the anal如cal processes in many fields，which includes pharmaceutlcal a11alysis，immunoassay，DNA analysis as well as clinical analysls·Green nanomat谢als are attractive in the developement of biosensors due to their sunace homogeneity of particle size，excellent electroconductibility and large speclnc sensors area as well as abundant functional groups，favorable biocompatibility·ECL ot based on盯een synthesized nanomaterials demonstrated very promlsmg propertles could signal ampliftcation labels in electrochemical applications·Funhemore，they conjugate with bioproteins and biomolecules． In this paper， carbon nanospneres fCNPs)，Ag@BSA microspheres(Ag@BSA)and Flower。like Au@BSA nalloparticles(Au@BSA)were used for the construction of ECL biosensors·The major contents displayed as fllows： f 1 1 In this work，we have frabricated a novel electrochemiluminescence(ECL)dmg sensor for sensitive atropine determination based on Ru(bpy)32+／carbon nanospheres／Nafion composite film．The carbon nanospheres(CNPs)with hlgh surface area、good electrical conductivity and favorable biocompatibility comDosite film were obtained through hydrothermal method·MoreoVer，owmg tO ion-exchange and membrane building feature of nation，we used nation to immobilize Ru(bpy)32+，resulting in abundant adsorption of Ru(bpy)3p·As result, the as．pr印ared ECL sensor Can be used to quantitative detection of atroPm Moreover，the ECL sensor is able to be used to detect atropine in real samples wltn for satisfying recovery．This preliminary method provided an effective strategy atropine detection，which showed promising applications drug a11alysls’ for (2)In the protocol，we developed an effective sandwiched ECL immunosensor CA 1 9．9 ultrasensitive