玉米种质资源亲缘关系分子标记鉴定和其耐盐性评价.PDF

中国农学通报 2013,29(33):79-84 Chinese Agricultural Science Bulletin 玉米种质资源亲缘关系的分子标记鉴定及其耐盐性评价 冯 艳，江 鹏，王亚峰，李金贝，吴雪玲，汪保华，李 平 （南通大学生命科学学院农业部南方平原地区玉米科学观测站，江苏南通226019 ） 摘 要：为鉴定10份玉米材料的亲缘关系，测试其耐盐性强弱并初步探讨玉米耐盐的生理机制，采用20 对SSR 引物对10份玉米材料的DNA 进行PCR 扩增，采用NJ 法进行聚类分析，构建亲缘关系图；同时分 别配置浓度为0、100、200、300 mmol/L 的NaCl 溶液，对幼苗进行盐胁迫实验，并测定株高作为耐盐性的 指标；7 次盐处理后，对抗氧化物酶包括超氧化物歧化酶和过氧化物酶活性进行了鉴定。根据分子标记 的结果初步鉴定出了10 个玉米材料间的亲缘关系，10份材料的遗传相似系数变化范围为0.54~0.71 。 结合株高和酶活的测定结果筛选出了耐盐性较强的玉米材料5 份，分别为‘先玉335 ’、‘郑单958 ’、 LH214、83IBI4 和LH204 。研究结果对于玉米耐盐育种的亲本组合选配具有一定意义。 关键词：玉米；聚类分析；耐盐性；酶活性；株高 中图分类号：S513 文献标志码：A 论文编号：2012-3708 Identification of Genetic Relationship of Corn Germplasms with Molecular Markers and Evaluation of Their Salt Tolerance Feng Yan, Jiang Peng, Wang Yafeng, Li Jinbei, Wu Xueling, Wang Baohua, Li Ping (ScientificObservingandExperimentalStationofMaizeinPlainAreaofSouthernRegion, MinistryofAgriculture,SchoolofLifeSciences,NantongUniversity, Nantong Jiangsu 226019) Abstract: In order to analyze the genotypes of 10 corn accessions, detect their salt-tolerant level and reveal salt-tolerance mechanism in corn, 20 pairs of simple sequence repeat (SSR) molecular markers were used to amplify the 10 corn accessions, and the corresponding SSR fingerprints were obtained to collect 0, 1 data. The method of NJ cluster analysis was performed to construct dendrograms. Meanwhile, NaCl solution with the concentration of 0, 100, 200 and 300 mmol/L, respectively was prepared and employed on the corn seedlings. Plant height of the corn materials was measured as the reference data of salt resistance. After seven times of treatment with NaCl solution, the activity of antioxidative enzymes including superoxide dis