中华花鳅外周血细胞形态观察.pdfVIP

中华花鳅外周血细胞形态的观察 高泽霞 王卫民 （华中农业大学水产学院，华中农业大学农业动物遗传育种与繁殖教育部重点实验室，武汉，430070) 摘要：报道了中华花鳅外周血细胞的显微结构。应用常规血涂片 Wright 和 Giemsa 染液联合染色， 在油镜下观察中华花鳅的外周血细胞。结果表明：中华花鳅的外周血中可区分出下列各种类型的血 细胞：红细胞、嗜中性粒细胞、嗜酸性粒细胞、单核细胞、淋巴细胞和血栓细胞，还可见到幼红细 胞、正在分裂的红细胞和正在分裂的血栓细胞。白细胞中血栓细胞和嗜中性粒细胞较多；嗜酸性粒 细胞体积最大，血栓细胞体积最小。 关键词：中华花鳅；血细胞；显微结构 Observation on the Peripheral Blood Cells Morphology of Chinese spined loach, Cobitis sinensis GAo Ze-xia, WANG Wei-min (Fisheries College, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education ，Huazhong Agriculture University, Wuhan 430070, China) Abstract: The morphology of the peripheral blood cells of Chinese spined loach (Cobitis sinensis) has been studied by light microscopy. Blood sample was collected from 10 fish by caudal vein, smeared and stained with Wright’s-Giemsa fluid. Observation of the peripheral blood cells using the microphotography showed that there were six types of cells including erythrocyte, neutrophil granulocyte, eosinophil granulocyte, monocyte, lymphocyte and thrombocyte. The immature erythrocytes were present in peripheral blood. Amitosis was observed in both erythrocyte and thrombocyte. The thrombocyte and neutrophil granulocyte are abundant among leucocyte, while eosinophil granulocyte is the biggest, and thrombocyte, the smallest. Keywords: Cobitis sinensis; bloods cells; microstructure 鱼类血细胞是机体免疫的重要成分，鱼类血细胞的研究始于l9 世纪末，以后，随着养殖事业以 及鱼类生理学、生态学、病理学和病毒学的发展，鱼类血细胞的研究也有了相应的进展。关于鱼类 [1] [2] [3] 血细胞形态结构的研究，国内外已有不少资料，如鲮鱼、鳙鱼、鲩鱼、鲢鱼 、鳗鲡 、鳜鱼 、黄 [4]