传染病病六原诊断技术.pptVIP

A Novel Coronavirus Associated with Severe Acute Respiratory Syndrome virus-isolation techniques electron-microscopical histologic studies Molecular and serologic assays Primer pair IN-2 (+) 5GGGTTGGGACTATCCTAAGTGTGA3 IN-4 (–) 5TAACACACAACICCATCATCA3 conserved regions of open reading frame 1b A 405-nucleotide segment The Genome sequence of the SARS-associated coronavirus Science 300 (5624), 1399-1404 (2003) Virus isolation ( bronchoalveolar lavage specimen) Viral particles were purified, genetic material (RNA) was extracted RNA was converted to cDNA by random-priming and oligo(dT) primi