藻源型溶解性有机氮的产生及不同时期藻类有机物的特性.PDF

中国环境科学 2015,35(9) ：2745~2753 China Environmental Science 藻源型溶解性有机氮的产生及不同时期藻类有机物的特性 古 励 1*,郭显强 1,丁昌龙 1,何 强 1,单志俊 2 (1.重庆大学,三峡库区生态环境教育部重点实验室,重庆 400045 ；2. 中国海诚工程科技股份有限公司,上海 200031) 摘要：研究了铜绿微囊藻藻细胞全生命周期中氮的转化过程以及藻类有机物的性质,结果表明:溶解性有机氮(DON)在藻类生长周期中总体 7 7 呈现上升趋势,藻细胞在其生长初期释放量小于 0.05mgDON/10 cells,衰亡期释放能力约为 0.40mgDON/10 cells;胞外有机物(EOM) 中分子 量分布在对数期和衰亡期以1kDa 和100kDa 为主,稳定期以30kDa 为主;胞内有机物(IOM) 的分子量分布随藻的生长变化较小,集中在 1kDa 和100kDa 范围内.三维荧光光谱分析表明,EOM 中荧光物质主要为类腐植酸和溶解性微生物代谢产物;而 IOM 主要由类芳香蛋白 和溶解性微生物代谢产物组成. 关键词：铜绿微囊藻；氮转化；溶解性有机氮 中图分类号：X524 文献标识码：A 文章编号：1000-6923(2015)09-2745-09 Formation of algae-derived DON and characterization of algae organic matter (AOM) from different stages. GU 1* 1 1 1 2 Li , GUO Xian-qiang , DING Chang-long , HE Qiang , SHAN Zhi-jun (1.Key Laboratory of Eco-Environment of Three Gorges Region of Ministry of Education, Chongqing University, Chongqing 400045, China ；2.China Haisum Engineering Co. Ltd., Shanghai 200031, China). China Environmental Science, 2015,35(9)：2745~2753 Abstract ：Formation rules of dissolved organic nitrogen (DON), as well as characteristics of extracellular oganic matter (EOM) intracellular organic matter (IOM) during the growth of Microcystis aeruginous have been studied. The results show as follows: DON concentration of EOM shows a growing tendency during the growth of Microcystis aeruginous, the yield of which is less than 0.05mg DON per 107 7 cells in the early growth phase, around 0.40mg DON per 10 cells in the late growth phase, respectively; molecular weight distributions of EOM during exponential phase and