仿刺参体壁中抗菌肽的分离与抑菌活性_孟春英.pdf

2016, Vol.37, No.07 33 1,2 2,3 2,3, * 2 2 2 1,2 1. 200000 2. 264006 3. 264006 5 % Escherichia coli Salmonella eternitidis Staphylococcus aureus Bacillus subtilis Tricine- - Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresisTricine-SDS 115 kD510 kD1 kD Tricine-SDS 4.35 kD Isolation and Activity of Antimicrobial Peptide from Body Wall of Apostichopus japonicas 1,2 2,3 2,3, * 2 2 2 1,2 MENG Chunying , WANG Maojian , ZHANG Jian , WANG Gongming , LIU Xin , JING Yuexin , WANG Ting (1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 200000, China; 2. Shandong Marine Resource and Environment Research Institute, Yantai 264006, China; 3. Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Yantai 264006, China) Abstract: The antimicrobial peptide was obtained from the body wall of Apostichopus japonicas and its activity was evaluated. The crude peptide was extracted from the body wall with 5% acetic acid and subjected to bioassay-guided fractionation for antimicrobial activity. The purification was completed by ultra-filtration and chromatographic separation with macroporous resin. A turbidimetric method was applied to evaluate the inhibitory activity of each sample against bacteria including Escherichia coli, Salmonella eternitidis, Staphylococcus aureus and Bacillus subtilis. By using Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresi