具有降胆固醇能力人源乳酸菌筛选摘要.pdf

现代食品科技 Modern Food Science and Technology 2014, Vol.30, No.3 具有降胆固醇能力的人源乳酸菌筛选 陈大卫，郭飞翔，顾瑞霞，邹东昊，赵海晴 （扬州大学食品科学与工程学院，江苏省乳品生物技术与安全控制重点实验室，江苏扬州 225127） 摘要：分离筛选出长寿人群肠道中具有益生功能的乳酸菌，并研究其体外降解胆固醇能力。结果表明分离获得的 80 株乳酸菌中， 9 株菌在模拟胃液中培养 3 h 的存活率较高，分别为26.60%、24.30%、22.50%、16.72%、46.61% 、16.10%、19.80%、19.60%、17.80%， 在 0.30%、0.50%及 1.00%的胆盐培养基中存活率最高的菌株为o2，存活率分别为21.31%、13.35%及 12.43%；9 株菌对氨苄青霉素、 青霉素 G、氯霉素敏感，对四环素、利福平、环丙沙星敏感或中度敏感；9 株菌对三株致病菌均有抑制作用，其中 V9 、o2 分别对沙 门氏菌、金黄色葡萄球菌抑制能力较强，抑菌圈直径分别为12.00 mm、11.40 mm，9 株菌对大肠杆菌的抑制作用普遍较强，抑菌圈 直径均大于 7.00 mm；9 株菌中 f10、o2、T1 及 V9 的胆固醇降解率较高，分别为45.85% 、40.76% 、41.70%、42.87% ，经生理生化试 验及 16S rDNA 测序鉴定分别为屎肠球菌、食窦魏斯氏菌、干酪乳杆菌及发酵乳杆菌，是良好的辅助降血脂候选菌株。 关键词：筛选；长寿人群；功能；乳酸菌；胆固醇 文章篇号：1673-9078(2014)3-114-120 Screening of Cholesterol-reducing Lactic acid bacteria from Human Origin CHEN Da-wei, GUO Fei-xiang, GU Rui-xia, ZOU Dong-hao, ZHAO Hai-qing (Jiangsu Province Key Laboratory of Dairy Biological Technology and Safety Control, College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China) Abstract: Lactic acid bacteria (LAB) with probiotic were isolated and screened from intestinal tract of longevity crowd, and the ability of cholesterol-reducing in vitro were investigated. The results showed that nine of eighty strains had high survival rates in simulated gastric juice for 3 hours, being of 26.60%, 24.30%, 22.50%, 16.72%, 46.61%, 16.10%, 19.80%, 19.60% and 17.80%, respectively. The survival rates of o2 were the highest, which were 21.31%, 13.35%, 12.43% respectively in 0.3%, 0.5% and 1.0% bile salt. Nine strains were sensitive to ampicillin, penicillin G, chloramphenicol, and sensitive or moderately sensitive to tetracycline, rifampicin and ciprofloxacin. They had bacteriostatic activity against Salmonella, Staphylococcus aureus and Esc