利用PCR_DGGE方法探究添加复合菌剂对堆肥微生物群落影响.pdf

农业环境科学学报 2011,30(12):2602-2607 Journal of Agro-Environment Science 利用PCR-DGGE 方法研究添加复合菌剂对 堆肥微生物群落的影响 王 晶，许修宏* （东北农业大学资源与环境学院，哈尔滨 ） 150030 摘 要：为研究接种复合菌剂对堆肥微生物群落变化的影响，以奶牛粪便和稻草为原料进行堆肥试验，设添加复合菌剂 （由 BLD 3 株木质纤维素降解细菌组成，经测序鉴定分别为枯草芽孢杆菌、地衣芽孢杆菌、假单胞菌属中未鉴定种）和不加菌剂两个处理，利用 传统平板培养与聚合酶链式反应 变性梯度凝胶电泳（ ）方法相结合研究两种堆肥处理对微生物群落演替的影响。结果 - PCR-DGGE 表明，基于传统培养方式，微生物数量呈升高 降低 升高 降低的趋势，且细菌数量明显大于真菌数量； 图谱显示，两种堆肥 - - - DGGE 处理的条带数呈现与传统培养相似的变化趋势。接种菌株在堆肥初期成功定殖，高温期成为优势菌株，降温期优势逐渐减弱。接种 菌剂增加了堆肥中细菌数量，提高了堆肥微生物群落多样性，从而促进堆肥微生物群落演替，缩短堆肥腐熟时间。 关键词：堆肥；微生物群落； PCR- DGGE 中图分类号： 文献标志码： 文章编号：1672- 2043(2011)12- 2602- 06 S141.4 A Effects of Adding to Complex Bacteria on Microbial Community of Compost Assessed by PCR-DGGE Tech－ niques WANG Jing, XU Xiu-hong* （ ） College of Resources and Environment, Northeast Agriculture University, Harbin 150030, China ： Abstract In order to study the influence of inoculated strains on composting microbial community strcture, composting experiment was con－ （ ducted, which used the cow dung and straw as raw materials. Two groups were designed, bacterial strains were added to one group code name as BLD, which were composed of three kinds of strains, and sequencing showed that they were B. subtilis, B.licheniformis and Pseu－ ） domonas , the other was the control. Variation of microbial communities in inoculation and