应用pcr-dgge分析添加菌剂对关上堆肥微生物群落多样性的影响.docxVIP

Classified Classified Index： Code：10224 Confidential(yes／no)．no NO．：120210039 Dissertation for the Master Degree Effects of Microbial Inoculum on Microbial Diversity during Composting Process analyzed by PCR-—DGGE Candidate：Siqi Li Supervisor：Professor Xiuhong Xu Degree Category：Master of Science College：College of Life Science First level discipline：Biology Second level discipline：Microbiology Harbin China June 2015 万方数据 墒重 墒重 目录㈣7277㈣21 28 摘昙要 i I。 万方数据 东北农业大学理学硕士学位论文3．1．4堆肥碳氮比的变化 东北农业大学理学硕士学位论文 3．1．4堆肥碳氮比的变化 1 7 3．2堆肥木质纤维素的变化 17 3．3堆肥中木质纤维素酶活变化研究 18 3．4应用DGGE方法分析堆肥中微生物群落多样性 ．．19 3．4．1堆肥样品总DNA的提取纯化 。19 3．4．2堆肥细菌群落多样性 20 3．4．3堆肥真菌群落多样性分析 26 3．4．4堆肥放线菌群落多样性分析 3 1 4讨论 ．．37 4．1堆肥理化性质的变化 ．．37 4．2堆肥中木质纤维素降解及酶活变化情况 ：： ．．37 4．3堆肥过程中微生物的多样性 ．．38 5结论 ．．41 致谢 ．． ．42 参考文献 ．43 攻读硕士学位期间发表的学术论文 ．．： 49 II 万方数据 CONTENTSCoNTENTS CONTENTS CoNTENTS Abstract in Chinese ． ．I Abstract in English 。 。 。 ．III 1 Intronduction ．． ． ． ．1 1．1 Overview of livestock and poultry dung microorganisms ．1 1．2 Compost microbial research status 1 1．2．1 Bacteria ． ． ．1 1．2．2 Fungi ． 2 1．2．3 Actinobacteria ： ．2 1．3 Research ofthe effect compost with exogenous inoculum ．3 1．3．1 Composting technology with single inoculum 3 1．3．2 Composting technology with Compound inoculants 3 1．3．3 Composting technology with extracellular enzymes ·4 1．4 Degradation ofLignocellulose in the compost ．．4 1．4．1 Composition and physical degradation of lignocellulose ．．4 1．4．2 Influencing factors of ligninocellulose degradation ．4 1．4．3 Lignocellulose degradation enzyme ．4 1．4．4 Bacterial community ofLignocellulose degradation ． 5 1．5 Method with microbial diversity in the compost 6 1．5．1 Tradkional method ． ．。6 1．5．2 Molecular biology techniques method 6 1,6 Purpose and signification ofresearch ． ． 7 2 MateriaIs and Methods ．9 2．1 Test materials ．． ．．一 一． ．． 一一一．一 ．。 ． 9 2．I．1 Composting Materials ．9 2．1．2 Compost inocualted and sampling ．9 2．1．3 Reagents and equipment ．9 2．2 Test methods ．．10 2．2．1 Cultured strain ． ．