黄山种自然杂交后代叶片解剖结构变异特性研究-中国农学通报.PDFVIP

中国农学通报 2012,28(10):209-212 Chinese Agricultural Science Bulletin ‘黄山种’自然杂交后代叶片解剖结构变异特性研究 王 玉，张金霞，丁兆堂 （青岛农业大学茶叶研究所，山东青岛266109 ） 摘 要：为研究‘黄山种’自然杂交后代叶片的抗寒性变异，筛选抗寒茶树种质资源，对204 份‘黄山种’自 然杂交后代种质材料叶片解剖结构进行了系统地鉴定与评价。采用徒手切片法，观测叶片总厚度、角质 层厚度、上、下表皮厚度、栅状组织层次和厚度、海绵组织松紧度和厚度等，并根据测定结果计算有关比 值。结果表明，‘黄山种’自然杂交后代单株叶片的组织结构差异较大，平均遗传多样性指数达到1.69 ， 平均变异系数达到23.69% ，变异类型比较丰富，在很多成分上变异系数较高，且符合抗寒组织特征。通 过叶片解剖结构指数进行聚类分析，发现前3 类种质材料的抗寒性较强。因此，可以利用‘黄山种’自然 杂交后代单株在叶片解剖结构上的变异特性，初步筛选茶树抗寒种质材料。 关键词：茶树；‘黄山种’；解剖结构 中图分类号：S6 文献标志码：A 论文编号：2011-3097 Research on Leaf Anatomical Structure of the Natural Hybrid Progenies of Camellia sinensis ‘Huangshanzhong ’ Wang Yu, Zhang Jinxia, Ding Zhaotang (Tea Research Institute of Qingdao Agricultural University, Qingdao Shandong 266109) Abstract: The aim was to the cold resistance variation of leaves in the natural hybrid progenies of ‘Huangshanzhong ’. 204 natural hybrid progenies of ‘Huangshanzhong ’tea plants (Camellia sinensis L. O. Kuntze) were systematically detected and evaluated to collect the cold resistance tea germplasm. The total thickness, horny layer thickness, upper under layer epidermis thickness, palisade tissue layering thickness and spongy tissue tightness thickness were observed by freehand section. And the ratios were calculated according to the determined results. The results showed that the leaf anatomical structure diversity and variation were significant in the natural hybrid progenies of ‘Huangshanzhong ’tea plants. The average diversity index and coefficient of variation were 1.69 and 23.69%, respectively. Cold resistance was accorded with most of a