玉米灰斑病菌生物学特性探究.pdf

植物病理学报 ACTApHYIOPATHOI／)cICASINICA33(4)：292—295(2003) 玉米灰斑病菌生物学特性研究 张益先，吕国忠，梁景颐，杨红，白金铠 (沈阳敷,Ik大学植保系，沈阳110161) and Tehon 摘要：玉米灰斑病菌(Cercosporazeae．nta)，d／s +蔗糖20 6～8，适宜温度为20—25％。病菌对葡萄糖、麦芽糖、乳 g和Richard培养基。适宜菌丝生长的pH4～12，最适宜的pH 糖和对酵母膏、硝酸钾、牛肉膏的利用好于其它碳源或氮源。分生孢子萌发的温度为20～30％．pH范围广，RH≥81％以上分生孢 子萌发，随湿度增加萌发率增高。营养对孢于萌发无影响。 关键词：玉米；灰斑病；培养基 characteristicsof ZHANG Yi—xian，LU Biological Cercosporazeae-mayd／s Guo—zhong，LIANG Jing-yi， YANG 110161。(Lit-a) Hong，BAIJin—kal(：‰mslyangAgritxdtumluniveⅢw，Sheayang Abstract：Inl-G∞ent leaf ofcolncaused TehonandDonielsbecamemore yearsgray spot byCercospora。Bc一mayd／s prevalent iIlthe amaof northernChina．The characteristicshavebeen ot maize-growing biological ofpathogen investigated．One opti． mlMTImediafor is leafdisk growth medium，inferiorPDA，V8 CercosporaⅫ·mayd／3peanut juieeagar，V8juiceplusSUgcal and carbonand and Richard’smedium．Amongthetestednitrogenresources，咖cose，maltose，lactoseyeastextract，{(N03 has110 andbeefextractamuseful selectiveeffect011conidial ofthe types．nutrition germination．Forgrowth pathogen、the is4—12withthe 6—8．thefavorable iSbetw∞n20—25t．Thesuitable favorable temperature pH optimumpH tempera