肝再生增强因子在+hbv相关慢加急性肝功能衰竭中的表达与其启动子区基因多态性分析.pdf

关键词：乙型肝炎病毒；ACLF ；肝细胞再生增强因子；单核苷酸； 多态性 3 Analysis of the single nueleotide PolymorPhisms augmenter of liver regeneration promoter region with ACLF Abstract objective: 1)To analyze value of clinical the patients suffering HBV infection that had different liver damaged using ELLISA method.2)To obtained allele frequency the SNP site of ALR gene promoter in Han population.3)To screened SNP site of ALR gene promoter with ACLF.4 ）To detected ALR serum concentration difference in SNPs among the different genotypes of ACLF. Methods : The patients HBV-related liver diseases more than 368 in-patient or the clinical were collected from 2010 to2013,including CHB(n=110)ACLF(n= 138) and healthy blood donors(n=120). Each groups collected blood were distributed into blood cells and plasma frozen at -20 ℃ to stored.1)ELLISA: To selected patients serum of each group and in-patient serum of ACLF .To observed Elisa values each group.2)Blood DNA extraction: EDTA anticoagulated blood samples were placed in a 37℃ incubator rapidly dissolved. extracted genome DNA using the QiAamp DNABlood.Concentration and purity were detected with spectrophotometer.3)Electrophoresis and PCR: to extracted and detected sample of DNA put into 1.5% agarose gel were subjected to electrophoresis in the elcectrophoresis buffer, finally the results observed in the ultraviolet transillumination. Followed by PCR products were send to invitrogen company 4 to sequence. 4). We determined to judge genetype and to analysis statistically significant different.To analysis correlation between genetype and ALR expression ；To screene