高盐环境下微生物群落结构与多样性研究海洋生物学专业论文.docxVIP

ABSTRACTHvpersaline ABSTRACT Hvpersaline enviromems include saline lakes and solar saltworks，in wllich water salinity is usually higher thaIl seawater．The organisms liVing in this enVironment are usually halo—toleraJlt aJld halophilic．The hypersaline and hyper-osmotic condition haVe resulted in the unique ceU stmcture，physiological如nctions and metabolic mechanism of the halophiles as well as biological community stmcture．Research on microbial communlty strucnlre aIld diVersity in hypersaline enVironment haS import趾t theoretlcal slgnlilcaIlce and research value in aspect of 1ife origin，bi0109ical adaptation mechanisms，aquaculnlre， environmental protection and biotechn0109ical utilization． This thesisstudied the diversity and structureof the bacterial community in brine of Bohai Bav solar saltworks and the bionocs inaritificial 4r纪m妇culutre system using DGGE techniaues．Brine water wim dif．ferent salinities was sampled矗om dif．ferent saltponds of HaIlgu sal帆orks in different seasons．The effects of the salinity，temperature and other physio．chemical par锄eters ofbrine on microbial diVersity aJld co删mull竹stnlctllre were inveStigated．In a110ther study，me effects of．aeration，salinity，c打bon supplementation and microbiolo画cal management on microbial community stmcture and diVersi够。氐he bionocs werestudiedinartificial彳厂把所砌culnlresystem． The resultsshowed that the rich microbial diversity in Bohai Bay solar saltworks were contributed bv the variation of t11e enviroIⅡnentaI salinity and temperature，as weU as high level of nutrient eutrophication in Bohai Sea．The relatiVely higher rallge-weighted ricllIless f附1 index’Sh锄on．Wiener index(H’)and Pielou eve皿ess index(J)observed in thjs studv renected the hi幽level of richness，biodiversity and eVe肿ess presented m the salt ponds，indicating that it has relatively stable ecosystem and Stronger carrymg capaClty· Both tempemture and salinity a丘乱ted bacterial biodiVersity in saltpond ecosystem，m vmich temper砷Jre had stronger ef!fect t11aIl sali