金钗石斛低聚糖纯品对乳杆菌抗应激能力的影响.PDFVIP

安徽农业大学学报, 2017, 44(6): 968-972 Journal of Anhui Agricultural University [DOI] 10.13610/ki.1672-352x025 网络出版时间：2017/12/14 14:37:23 [URL] /kcms/detail/34.1162.S1437.050.html 金钗石斛低聚糖纯品对乳杆菌抗应激能力的影响 1.2 1,2 1,2* 渠 婷 ，马朝阳 ，王洪新 (1. 江南大学食品学院，无锡 214122 ；2. 国家功能食品工程技术研究中心（江南大学），无锡 214122) 摘 要：利用水作为溶剂从金钗石斛中提取糖类成分，经无水乙醇沉淀脱蛋白质和多糖，再经大孔树脂分离， 得到的低聚糖通过葡聚糖凝胶柱，获得 1 个低聚糖纯品。将纯品冷冻干燥后，添加到乳杆菌的培养基中，分别对乳 杆菌的生长曲线、耐热性、抗苯酚抑制作用进行了实验研究，结果表明，金钗石斛低聚糖在促进乳杆菌生长、降低 高温对于乳杆菌的伤害和苯酚对于乳杆菌的伤害方面都有明显效果。 关键词：金钗石斛；低聚糖；纯化；乳杆菌；抗应激能力 中图分类号：TS201.2 文献标识码：A 文章编号：1672352X (2017)06096805 Isolation of oligosaccharide from Dendrobium nobile and it effect on the anti-stress ability of Lactobacilli QU Ting1,2, MA Chaoyang1,2,WANG Hongxin1,2 (1. School of Food Science and Technology , Jiangnan University , Wuxi 214122; 2. National Engineering Research Center for Functional Food, Wuxi 214122) Abstract: Dendrobium Nobile oligosaccharide was extracted in water and purified by a three-step process: precipitation of protein and polysaccharides by anhydrous ethanol, decolorization after precipitation with macroporous resin and final purification through Sephadex G-25 gel chromatography. As a result, a resultant oli- gosaccharide compound was obtained, and its effect on growth curve, heat resistance and anti-phenol inhibition of Lactobacilli in a stress environment was further studied. The results showed that the oligosaccharide significantly promoted the growth of Lactobacilli, and reduced the damage caused by high temperature and phenol. Key words: Dendrobium nobile ; oligosaccharide; isolation; Lactobacilli; anti-stress ability