银沙槐内生放线菌抗菌活性及其与内生细菌的拮抗关系-应用生态学报.PDF

应 用 生 态 学 报摇 2010年4月摇 第21卷摇 第4期摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 ChineseJournal of Applied Ecology,Apr.2010,21(4):1021-1025 银银沙槐内生放线菌抗菌活性及其与 内生细菌的拮抗关系* ** 安登第 摇 陈玉梅摇 李摇 进摇 朱艳蕾摇 迪丽拜尔·托乎提 (新疆师范大学生命科学与化学学院濒危物种保护生物学重点实验室,乌鲁木齐830054) 摘摇 要摇 从荒漠濒危植物银沙槐根部分离到内生放线菌12 株和内生细菌31株. 以大肠杆 菌、金黄色葡萄球菌和枯草芽孢杆菌为指示菌对内生放线菌进行了抗菌活性筛选.结果表明: 3株放线菌对3 种指示菌均有抑制作用,2株完全无抑菌作用.采用菌块抑制法分析内生放线 菌对来自同一植物内生细菌的抑制效果,44郾9% 的组合出现抑制现象,最大抑菌圈直径为 28郾5 mm,最小者仅8郾25 mm,主要抑制对象是革兰氏阴性菌和芽孢菌;55郾1%的组合无抑制 现象.说明内生菌的相互作用具有多样性. 关键词摇 内生放线菌摇 抗菌活性摇 内生细菌摇 银沙槐 文章编号摇 1001-9332(2010)04-1021-05摇 中图分类号摇 Q936摇 文献标识码摇 A Anti鄄microbial activity of endophyticactinomycetesisolatedfromAmmodendronbifolium and their antagonism to endophytic bacteria. AN Deng鄄di,CHEN Yu鄄mei,LI Jin,ZHU Yan鄄lei, DILBAR ·Tohty (Key Laboratory of Rare and Endangered Species Conservation Biology,College of Life Science and Chemistry,Xinjiang Normal University,Urumqi 830054,China).鄄Chin.J.Appl. Ecol. ,2010,21(4):1021-1025. Abstract:Atotal of twelve endophytic actinomycetesandthirty鄄one endophyticbacteriawere isola鄄 ted from the surface鄄sterilizedAmmodendron bifolium (Pall.) Jakovl roots,and the anti鄄microbial activity of the endophytic actinomycetes was screened,taking Escherichia coli,Staphylococcus au鄄 reus,andBacillussubtilis astheindicator strains. Threeofthe 12 isolatedendophyticactinomycetes could inhibit the growth of the three indicators,and two did not show any anti鄄bacterial activity. The antagonism of the endophytic actinomycetes against the endophytic bacteria isolated from same plane wassignificant different. About44郾9% pairing showed inhibiting activity,with the diameters of inhibitionzonefrom8郾25 mmto28郾5 mm,andth