三倍体虹鳟卵巢败育的调控机制研究畜牧学动物遗传育种与繁殖专业论文.docxVIP

CoNTENTSCoNTENTS CoNTENTS CoNTENTS Abstract in Chm髓e ． ．1 Abstract in English V l Introduction ． ． ． ． ． ．．1 1．1 Fish sex determination and differentiation ． ． ． ． 1 1．1．1 Hermaphroditic fish sex determination mechanism 。3 1．1．2 Fish gonad differentiation ． ． ． ．3 1．2 The hypothesis of gonad differentiation and aromat硒e 。7 1．2．1 Germ cell proliferation and estrogen production is related to ovary differentiation 。7 1．2．2 The potential relationship between germ cell proliferation，estrogen production and ovary differentiation 8 1．3 Sex determination is multiple gene regulation cascade 9 1．3．1 The master regulating genes involved in differentiation ．9 1．3．2 The Cyp l 9al a upstream gene regulation feedback loop during female differentiation．1 1 1．3．3 Androgen physiological effect and gene regulation cascades of male male differentiation ．1：1 1．4 Research about gonadal development and reversal offemale triploid rainbow trout ．．13 1．4．1 Gonadal development of female triploid rainbow trout 13 1．4．2 Sex reversal of all-female triploid rainbow trout ．14 1．5 The significance and major contents ofthis study ．．14 1．5．1 The significance ofthis study 。14 1．5．2 Major contents 15 2 Materials and Methods ．16 2．1 Experimental materials ．16 2．1．1 ExperimentaI fish ．．16 2．1．2 Breeding conditions and managements ofexperimental fish ．16 2．1．3 Experimental fish treated with exogenous hormone ．．17 2．1．4 Groups ofexperiment treated with exogenous hormone 。17 2．1．5 Sample collections and preparations ．1 8 2．2 Experimental methods ．．19 2．2．1 Ploidy detection about triploid rainbow trout．DNA content detection ．1 9 2．2．2 Cytogenetic analysis of female triploid rainbow