低磷胁迫对番茄根系生长及根际土壤微生物多样性的影响园艺学蔬菜学专业论文.docxVIP

达到显著或极显著相关。 达到显著或极显著相关。 (5)低磷胁迫处理显著改变了土壤细菌群落结构，导致部分诸如甲基 杆菌属(Methylobacterium sp．)等具有解磷功能的菌群缺失。同时，低磷胁 迫导致番茄根际土壤细菌多样性指数(H)、丰富度(S)和均匀度指数(Eh) 降低。并测序结果表明：低磷胁迫条件下，番茄根际土壤中的细菌种群主 要为不可培养细菌种属，但亦出现鞘脂单胞属(Sphingopyxis sp．)、甲基杆 菌属(Methylobacterium sp．)、金黄杆菌属(Chryseobacterium sp．)以及大 肠杆菌属(Escherichia coli sp．)等部分具有解磷功能的可培养细菌种属。 (6)低磷胁迫处理对番茄根际土壤真菌群落结构的影响与细菌相似。 低磷胁迫亦导致番茄根际土壤真菌多样性指数(日)、丰富度(S)和均匀度 指数(Eh)下降。测序结果同样表明：低磷胁迫条件下，番茄根际土壤中 优势真菌种属亦以不可培养真菌为主，同时亦包含部分具有解磷功能的青 霉属(Penicillium sp．)、镰刀菌属(Fusarium sp．)、曲霉属(Aspergillus sp．) 等可培养真菌种属。 综上所述，低磷胁迫条件下，番茄根际土壤微生物群落结构劣化和肥力 下降是导致番茄植株生长不良的根本原因。平衡施肥对番茄的正常生长和 提升土壤肥力以及维护土壤健康具有极其重要的作用。 关键词：番茄低磷胁迫根系土壤微生物多样性PCR．DGGE Ⅱ 万方数据 EFFECT EFFECT OF LOW PHOSPHoRoUS STRESS oN ROOT GROWTH AND SOIL MICRoBIAL DIVERSITY IN RHIZOPLANE oF ToMAToS ABSTRACT m 10mato lS one o士the most popular vegetables in me wofld．With the increase of the planting area of tomato，the nutritional deficiency and monocultural problems are getting more severe in tomato production area． During the process of tomato growth，phosphorus deficiency will lead to slow growth，flowering delay,etc．Based on the traditional and modem analyzed techniques，such as WinRHIZO root analysis system，dilution-plate method， PCR-DGGE etc．，the effect of low phosphorus stress on root growth，soil microbial properties and diversity under low phosphorus stress were analyzed． The main results are as follows： (1)The results showed that the root length，surface area，volume and tip numbers were all decreased under low phosphorus stress．But the effects of low phosphorus stress on root growth of different tomato cultivars were not consistent．Meawhile，the growth of the upper part of the tomato was also declined and the ratio of root—shoot significantly increased under low phosphorus stress． III 万方数据 (2)Soil (2)Soil pH，total phosphorus，potassium and available phosphorus contents in rhizosphere of tomato under low phosphere stress were significantly lower than those in balanced fertilization． (3)In contrast，Soil microbial n