三孢布拉氏霉菌产类胡萝卜素发酵调控机理的研究化学工程与技术专业论文.docxVIP

摘要角固醇的合成减少。麦角固醇是类胡萝b素合成途径的支路之一，也与类 摘要 角固醇的合成减少。麦角固醇是类胡萝b素合成途径的支路之一，也与类 胡萝卜素竞争进入细胞膜，它的减少有利于类胡萝卜素合成的增加。同时， 还发现ALA显著促进八氢番茄红素合成酶／番茄红素环化酶(carRA)和 八氢番茄红素脱氢酶(carB)基因的表达，其次是促进拢牛儿基栊牛儿基 焦磷酸合酶基因(carG)的表达。此外，还发现了与类胡萝b素合成有关 的转录因子Mot3的转录明显上调。Mot3是个多功能的调控因子，还具有 抑制麦角固醇合成，抑制响应性激素的基因的表达。Mot3在三孢布拉氏 霉菌中的作用，需要后续实验验证。一些转录因子的发现，表明ALA调 控孢子的形成，菌丝及细胞壁的生成，细胞的分裂及性发育，如SteA。 SteA是否也参与调控类胡萝b素的合成，需要进一步的验证。此外，还发 现了对过氧化氢及其敏感的转录因子PapI和附1的表达上调，这说明氧 化应激是类胡萝卜素合成增加的外界刺激因素之一。 关键词：三孢布拉氏霉菌，p一胡萝卜素，代谢组学，咖亚麻酸，RNA-seq 万方数据 ABSTRACTTHE ABSTRACT THE STUDY 0F REGULATIVE MECHNISM 0F CAROTENOID PRODUCION BY删KE趾上翻 TRISPoRA ABSTRACT Blakeslea trispora was found an excellent strain to produce 13-carotene in the level of industrialization．The way of carotenoids production by the microbial fermentation has the advantages of lower cost，high purity,without seasonal restriction、toxic or side effect．Previous researches indicated that the exogenous lipids could enhance the lycopene formation,especially the oils witll lligh content of linoleic and linolenic acid．Compared with the sunflower oil and soybean oil，our results showed that linseed oil had a beRer effect on promoting the carotenoids production．However,the mechanism how ALA promoted the carotenoid production was unknown，Linseed oil is rich in a-linolenic acid (ALA)，Thus，we chose ALA to study it how to promote carotenoid production at metabolism and transcription level by B．trispora． Wre studied the changes of intracellular metabolites induced by ALA addition by using gas chromatography-mass spectrometry(GC—MS)and multivariate analysis method in B．trispora．Based on the PLS。DA(partial least squares．discriminant analysis)method，we found that this method could separate the test group from the contr01．The results ofcluster analysis supported the reliability of PLS．DA．W_e found that the content of linolenic acid and linoleic acid were much higher than the con仃ol wil[h ALA treated．while there were no difference in the content of their precursors between幽e two groups． Fatty ac