cds纳米粒子功能化及其在分析中的应用研究分析化学专业论文.docxVIP

约为20 约为20 nlTl。CdS纳米微粒的表面经ZnS修饰后，表面缺陷减少， 其荧光发射峰强度显著增强。利用该探针对DNA进行了定量测 定，考察了各种因素的影响，在最佳实验条件下，该方法测出有 两个线性区间，分别为：0．0833—3．33 gg／mL和3．33～l 6．7 gg／mL， 该方法的检出限为2．50×10一gg／mL。与其它方法相比，该方法具 有简单、经济、快速、灵敏度高、低毒的特点。 圆以已知序列的单核苷酸为对象，磁性纳米颗粒作为压电检测增敏 和磁分离材料，以单碱基修饰的CdS纳米粒子作为探针，利用压 电与荧光光谱分析，建立一种新型的基于纳米粒子的单核苷酸多 态性检测新方法。利用外加磁场的固定过程，成功地提供了一种 简便、快捷、廉价的检测装置。加入单核苷酸修饰的CdS纳米粒 子后，突变体系频率下降，而对应的完全互补目标DNA链，频率 下降可以忽略，这一结果由荧光光谱分析后得到进一步的证实。 因此，由于其操作简便且成本低，以上方法必将给研究领域和临 床基因分析带来福音。 关键词：CdS纳米粒子、CdS／ZnS、磁性纳米粒子、锰离子、DNA、 点突变、荧光光谱、压电 ABSTRACTNanostructured ABSTRACT Nanostructured semiconductor materials with unique optical and electrical properties have attracted a lot of interests in the last two decades．As a direct bandgap II-VI semiconductor，CdS nanoparticles have been paid more attentions for their excellent optical properties． Compared to the conventional organic dyes，CdS nanoparticles appear to be less susceptible to photobleaching with much narrower emission spectra and tunable maximum emission wavelength，which have been successfully used as fluorescent probe for the imaging of biological samples and cells．And the CdS nanoparticles become very sensitive to their local environment，and detections of some inorganic ions，proteins， DNA conformations were thus developed based on this property． On the basis of related literatures，the synthesization of highly luminescent water-soluble CdS nanoparticles was studied．We modified the CdS nanoparticles to be fluorescent probes to detect inorganic ion， DNA and single—base mutations．The main work in this thesis is as follows： (1)Reviews were given firstly on the properties，synthetic methods and applications of CdS nanoparticles． (2)CdS nanoparticles were synthesized by the aqueous solution method． The nanoparticles were of uniformity in size and good dispersancy． CdS nanoparticles were modified with cysteine and cysteamine， respectively．The factors those influencing the CdS nanoparticle fluorescence were tracked and inspected．The interaction mechanism between CdS