非酒精性脂肪性肝病大鼠肝组织microrna表达谱的分析内科学专业论文.docxVIP

The The Analysis on microRNA Express Spectrum of Rat’S Liver Tissues in Nonalcoholic Fatty Liver Disease Abstract objective：This study is aimed to offer some biological markers for intervention，diagnosis and treatment of nonalcoholic fatty liver disease in an early stage，as well as lay the foundation for further studies in the role of microRNAs in apoptosis．Expression of microRNAs spectrum was screened in rat’S liver tissues．Methods：After 1 week’S adoptive feeding，1 6 4-weeks—old weighing about 200 grams male SD rats were divided into control group (named E group)and model group(named Y group)randomly(8 rats in each group)．E group was fed with normal diet，while Y group was fed with high fat diet containing sugar and fat．The animal model of nonalcoholic fatty liver disease was established after 1 2 weeks．Then the animals were sacrificed and the liver tissues were rapidly taken out to do HE staining to observe the severity of fatty degeneration，inflammatory activity and calculate histological score．Then blood was drawn 0f!F from abdominal aorta and automatic biochemical analyzer was used to measure the level of aspartate aminotransferase，aspartate aminotransferase，total cholesterol，total triglyceride and fasting plasma glucose with enzymatic method．The total RNA of each fresh liver tissue was extracted with Triz01．After qualified with NanoDrop 2000 and Agilent Bioanalyzer 2 1 00，the sample was performed on microRNAs gene chip with GeneChip miRNA 4．0 to screen the differentially expressed miRNA，and the miRNA was verified with real—time PCR．Results：1、General ．3． 万方数据 observation：In observation：In the process of feeding，the animals of E group was live and spiritual with clean hair,and gained weight continually．On the other wise，the animal of Y group was dispirited and sluggish with dull hair,and gained weight slowly．2、The index of liver：Compared with E group，the weight of rat and liver in Y group were no obviously changed(PO．05)，while the liver index was increas