从雄烯二酮发酵废液中分离甾醇和油脂的工艺研究.docVIP

PAGE PAGE 10 从雄烯二酮发酵废液中分离甾醇和油脂的工艺研究基金项目：河南省科技攻关计划项目(172102310470 基金项目：河南省科技攻关计划项目(172102310470)； 收稿日期：2018-04-08 作者简介：王莹，女，1984年出生，硕士，工业微生物菌种的选育及应用，生物活性成分的分离、提取 通讯作者：刘凤霞，女，1960年出生，硕士，工业微生物菌种的选育及应用，生物活性成分的分离、提取 王 莹1,2 刘凤霞1,2,* 薛 刚1,2 娄源民3 何际芳1,2 （南阳理工学院生物与化学工程学院1，南阳 473004） （河南省工业微生物资源与发酵技术重点实验室2，南阳 473004） （河南恒天久大实业有限公司3，郑州 450001） 摘 要:以雄烯二酮发酵废液为原料，采用有机溶剂萃取法和重结晶的方法对原料进行处理，分离获得甾醇和雄烯二酮产品。通过单因素实验考察不同溶剂、不同配比、不同温度等因素对分离影响。研究结果显示：以乙酸乙酯为溶剂，按料液比（1:3）萃取温度50 ℃、萃取两次、结晶温度为0 ℃、结晶时间为6 h、结晶二次得到甾醇提取率为46%，纯度达到80%以上；按料液比1:3加入甲醇溶解浓缩物，浓缩到体积的一半后，冷却结晶18h，经离心得到雄烯二酮粗品。上清液再次浓缩，回收溶剂和副产品大豆油。该研究结果实现了雄烯二酮发酵废液的变废为宝及资源化综合利用。 关键词:发酵废液，萃取，甾醇，雄烯二酮，重结晶 中图分类号:Q815 文献标识码:A doi: Study on isolation of sterols and lipid from the androstenedione fermentation waste liquid Wang Ying1,2, Liu Fengxia1, 2, *, Xue Gang1,2, Lou Yuanmin3, He Jifang 1,2 (1. School of Biological and Chemical Engineering, Nanyang Institute of Technology, Nanyang 473004, China; 2. Henan Key Laboratory of Industrial Microbial Resources and Fermentation Technology, Nanyang 473004, China; 3. Henan Kingdo Industries Co. Ltd., Zhengzhou 450001, China) Abstract: In this paper androstenedione fermentation waste liquid was employed as a raw material. Organic solvent extraction and recrystallization were carrieded out on this research. Sterols and androstenedione products has been gained. Single factor experiment was used to invest the effect of different solvents, different proportions and different temperature on the separation . The results showed: ethyl acetate as the solvent, according to the solid-liquid ratio (1: 3) extraction temperature 50 ℃, extraction twice, the crystallization temperature is 0 ℃, crystallization time 6 h, crystallized twice to obtain sterol extraction rate of 46% purity more than 80%; by solid-liquid ratio of 1: 3 methanol was added to dissolve the concentrate, concentrated to half volume followed by cooling crystallization 18 h, centrifuged and the precipitate obtained androstenedione crude