BioOcularTM角膜上皮模型ET50法在化妆品体外眼刺激.PDF

BioOcularTM 角膜上皮模型 ET50 法在化妆品体外眼刺激评价中的应用  张劲松，桑晶，匡荣，洪利娅*(浙江省食品药品检验研究院，杭州 310052) 摘要：目的 通过 BioOcularTM 角膜上皮模型ET50 法评价不同配方的化妆品在眼刺激性上的强弱。方法 采用三维人源 重组角膜上皮模型 BioOcular™模型 ET50 法对不同品牌洗发水和面霜的眼刺激性进行比较研究，区分不同配方产品的眼 刺激性强弱等级。结果 在同样实验条件下，洗发水和面霜表现出对三维人源重组角膜不同的刺激程度，不同配方品牌 的洗发水的刺激程度也有很大差别。结论 采用三维人源重组角膜上皮模型的ET50 法是一种评价不同配方化妆品以及原 料的眼刺激性的差异的快速替代检测方案。 关键词：三维人源重组角膜；ET50 ；眼刺激 中图分类号：R965.3 文献标志码：A 文章编号：1007-7693(2017)06-0846-03 DOI: 10.13748/ki.issn1007-7693.2017.06.013 引用本文：张劲松, 桑晶, 匡荣, 等. BioOcularTM 角膜上皮模型ET50 法在化妆品体外眼刺激评价中的应用[J]. 中国现代应 用药学, 2017, 34(6): 846-848. Evaluation of Eye Irritation Potential of Cosmetics Using Human Cornea-like Epithelial Tissue Model BioOcular™ ET50 Method in Vitro * ZHANG Jinsong, SANG Jing, KUANG Rong, HONG Liya (Zhejiang Institute for Food and Drug Control, Hangzhou 310052, China) ABSTRACT: OBJECTIVE To evaluate eye irritation potential of different shampoos and facial creams using human cornea-like epithelial tissue model BioOcular™ ET50 method in vitro. METHODS Different shampoos and facial creams were tested in eye irritation ET50 test with reconstructed human cornea-like epithelial tissue model BioOcular™ in vitro, distinguish eye irritation levels of different formulations. RESULTS Under the same condition, shampoos and facial creams showed irritation at various levels in ET50 test with reconstructed cornea-like epithelial tissue model. Also different ingredients and different brand of shampoos showed different degrees of irritation. CONCLUSION It is a quick alternative way to use ET50 method to evaluate the irritation of different cosmetics and ingredients with reconstructed human cornea-like epithelial tissue model BioOcular™ in vitro. KEY WORDS: recon