成体雌性小熊猫卵巢组织学观察.PDFVIP

兽类学报,2010,30 (3):310 - 316 Acta Theriologica Sinica 成体雌性小熊猫卵巢组织学观察 修云芳 (福州大熊猫研究中心,福州 350001 ) 摘要:2000 年至2009 年,12 只固定于10% 福尔马林中非生殖系统疾病死亡的小熊猫卵巢组织,按常规组织学 技术制作组织切片,HE 染色,光学显微镜观察。结果:(1)不同发情时期卵巢均有原始卵泡、初级卵泡和次级 卵泡分布。发情期的卵巢未观察到典型的成熟卵泡和卵母细胞; (2 )原始卵泡数量较少,初级卵泡数量较多, 多数初级卵泡和大多数的次级卵泡都处在闭锁状态; (3) 卵泡腔出现之前,卵母细胞的直径和卵泡直径同时增 长;卵泡腔出现之后,卵母细胞直径增长较慢,卵泡直径增长较快; (4 )不同发情时期的小熊猫卵巢均存在大 量的间质腺细胞;(5 )妊娠小熊猫和发情间期无妊娠小熊猫的卵巢均有发育正常的黄体; (6) 卵泡细胞发育呈 低柱状至柱状时出现透明带。结论:(1) 卵泡闭锁主要发生在初级卵泡阶段,仅少数卵泡能发育至次级卵泡; (2 ) 卵母细胞和卵泡生长呈双相生长的趋势; (3) 不同发情时期的小熊猫卵巢间质腺都发达; (4 )发情排卵 后,非妊娠黄体与妊娠黄体维持的时间相似,证实了小熊猫存在假孕现象。 关键词:小熊猫;组织学;卵巢;繁殖 中图分类号:Q954 6 文献标识码:A 文章编号:1000 - 1050 (2010 )03 - 0310 - 07 Histological observations on ovaries of adult female red pandas (Ailurus fulgens ) XIU Yunfang (Fuzhou Giant Panda Research Center,Fuzhou 350001,China) Abstract :Twenty-four ovaries were collected from twelve adult female red pandas died of non-reproductive system diseases at different stages of estrous cycles from 2000 to 2009 The ovaries were fixed in 10% formalin,and tissue slices were made using standard histological techniques and stained with HE All slices were observed under optical microscopes Re- sults showed that (1)Primordial follicles,primary follicles and secondary follicles exist simultaneously in all ovaries of dif- ferent stages of estrous cycles Typical mature follicles and oocytes are not observed in the ovaries during the estrous stage (2 )There are more primary follicles than primordial follicles and most of primary follicles and secondary follicles are artre- sic in all ovaries (3 )Before follicular antrum formed,oocytes and follicles are developed synchronously,while in antral follicles,the development of fol