基于质谱技术的细胞代谢组学研究进展.pdf

中国细胞生物学学报 Chinese Journal of Cell Biology 2017, 39(8): 1130– 1134 DOI: 10.11844/cj cb.2017.08.0375 基于质谱技术的细胞代谢组学研究进展 汪思媛 赵星阳 徐玮蔚 刘慧雯* (哈尔滨医科大学组织学与胚胎学教研室, 哈尔滨 150081) 摘要 细胞代谢组学作为一个新兴领域, 能解决基本的生物学问题, 还能观察细胞内的代谢 情况。细胞代谢物浓度可以近似地反映一个组织、器官或细胞的表型。随着代谢组学的发展, 以 质谱分析为基础的代谢组学技术研究细胞的代谢物, 其灵敏度高、分辨率好, 能进行多组分的检 测, 并能获取分子的结构信息, 这有利于细胞生物学的研究。该文结合目前代谢组学的技术, 对细 胞代谢物研究的意义及基于质谱技术的细胞代谢组学的应用进行了综述。 关键词 细胞代谢组学; 质谱; 代谢物 Advances in Cell Metabolomics Based on Mass Spectrometry Wang Siyuan, Zhao Xingyang, Xu Weiwei, Liu Huiwen* (Department of Histology and Embryology, Harbin Medical University, Harbin 150081, China) Abstract Cell metabolomics is an emerging field that addresses fundamental biological questions and allows observation of metabolic phenomena in cells. Cellular metabolite concentrations closely reflect the phenotype of an organism, tissue, or cell. With the development of metabolomics, thetechnology of metabolomics which is based on mass spectrometry can be applied to study cell metabolites. And it has high sensitivity, good resolution, the capability for multi-species detection and molecular structure identification, which is significant in the research of cell biology. In this review, combining with the current technology of the metabolomics, the study significance of cell metabolites and the applications of cell metabolomics based on mass spectrometry are reviewed. Keywords cell metabolomics; mass spectrometry; metabolites 在后基因组时代, 生命科学研究已经进入全 中所有的小分子代谢物(分子量小于1 500 Da)[2] 。基 面、系统和动态的功能探索阶段。多种高通量研 因和蛋白质表达在功能水平上的微小变化可在代谢 究策略如基因组学、转录组学、蛋 白质组学和代 物上得到放大, 并且许多基因和蛋白质的非功能性 谢组学, 还有小RNA测序被组合应用到同一个课 变化不会在代谢物上反映出来,