低温离心净化.pdf

6 2 Vol. 6 No. 2 2015 2 Journal of Food Safety and Quality Feb. , 2015 II O 杨 爽, 俞子萱, 王永芳, 葛宝坤* (, 300461) 摘 要: 目的 II O -方法 70% - (0.5%) 4 ℃12000 r/min (high performance liquid chromatography, HPLC), (PDA), (Rt), 结果 0.00125~0.05 mg/mL , , 0.9999; 0.052.0 10.0 mg/kg 95.0%~103.0%(n=10), 5.0% (LOD)0.02 mg/kg, (LOQ)0.05 mg/kg结论 , , 关键词: ; ; ; ; II; O Rapid determination of chrysoidine II and auramine O in dried bean curd sticks by cryogenic centrifugal purification–high performance liquid chromatography * YANG Shuang, YU Zi-Xuan, WANG Yong-Fang, GE Bao-Kun (Animal , Plant and Foodstuffs Inspection Center, Tianjin Entry-Exit Inspection and Quarantine Bureau, Tianjin 300461, China) ABSTRACT: Objective To establish a method to detect chrysoidine II and auramine O in dried bean curd sticks by cryogenic centrifugal purification–high performance liquid chromatography. Methods The samples were diluted and extracted by 70% acetonitrile ammonia water, purified by centrifugation at 12000 r/min and 4 ℃, separated by high performance li