β2微球蛋白电化学发光免疫分析方法的建立生物工程专业论文.docxVIP

f12微球蛋白电化学发光免疫分析方法的建立Establishment f12微球蛋白电化学发光免疫分析方法的建立 Establishment electrochemical luminescence immunoassay analysis method for p2 microglobulin Abstract In Chma，Number of uremic patients have more than one million,132 microglobulin is a middle molecule weight of protein which exist in the uremia patients，In vivo long．term accumulation of p2 microglobulin will cause various complications in peripheral tissue， digestive tract and cardiovascular region．The concentration of陡microglobulin in healthy human serum is relatively stable，generally range on 1．5-3 mg／L．However，the concentration of 132 microglobulin in uremic patients Can reach 20-50 mg／L．and sometimes up to 1 00 mg／L． The aim of early diagnosis is early detection and treatment，through diagnose the signs of the disease，establishing the best treatment options，and then cure the patients．Early renal failure is a critical period for the patients．Therefore，it is very important to establish a convenient， rapid and high sensitive detection method to prevent the incidence of renal failure．In this paper，laboratory electrochemiluminescence detection platform was established，which is used to detect the 132 microglobulin．The research work is carried as followed： (1)The fabrication of magnetic working electrodes This paper established a new magnetic working electrode fabrication method,the magnetic working electrode conductivity and magnetic adsorption properties were tested．The results showed that the graphite rod electrode had high intensity of electromagnetic induction when the size was 5 mm in diameter，3 toni in hight，which up to 235．7 mT；Graphite electrode had a good electrical conductivity，and could capture magnetic microspheres efficiently． (2) Establishment and optimization of antibody-coated magnetic microspheres immobilization method Magnetic microspheres had large specific surface area，and a large amount of immobilized antibody，they can be separated rapidly in the external magnetic field．Using magnetic micr