高压电场诱变大肠杆菌k12的研究生物物理学专业论文.docxVIP

内蒙古大学硕士学位论文突变；且HVEF和HVFEF的间接作用也可能是导致细胞基因突变的原因之一。 内蒙古大学硕士学位论文 突变；且HVEF和HVFEF的间接作用也可能是导致细胞基因突变的原因之一。 关键词：高压静电场；高压变频电场；大肠杆菌；lacl基因；突变谱；诱变机制 II 万方数据 内蒙古大学硕士学位论文STUDY 内蒙古大学硕士学位论文 STUDY ON HIGH VOIJAGE ELECTRIC FIELD n旧UCED STRAIN OF ESCHERICHIA COLI K 1 2 AB STRACT In this study,the wil扣type Escherichia coli饵coli)K 1 2 W3 1 1 0 was the research object，The bacteria were treated by the high-voltage electrostatic field (HVEF)and high-voltage variable frequency electric field(HVFEF)，also with two control groups setting up，respectively：spontaneous group(CKl)and drying control group(CK2)．By examining cell survival rate，mutmion rate and morphological damage of the cell，to study the damage effect of E．coli by hi曲voltage electrostatic field effect；The mutant were screened from the treatment conditions with significant mutation rate(HVEF：4kWcm木2min；HVFEF：4kHz木4kWcm水6min)(HVEF： 4kV／cm木2 min；HVFEF：4kHz木4kV／cm木6min、)and draw mutation spectrum of E．coli lncI gene，research mechanism of lacI gene mutations induced by high voltage electric field． The experimental results show that： (1)HVEF treatment：With electron microscope，it Was found that the cell III 万方数据 内蒙古大学硕士学位论文membrane 内蒙古大学硕士学位论文 membrane was damaged and the cell comems were overflow,which caused by the effect of HVEE showed that HVEF can effectively destroy the cell membrane structure；By examine the changes of nucleic acid and protein content relative of the bacteria under the same field and different time，we found that the change of the above two parameters were oscillatory,showed that the HVEF could regulate the permeability of the cell membrane，and E coli itself may have the function of damage repair．Under the dose of 4 kWcm宰2 min，the mutation rate Was the highest， shows(13．73+1．99)木10一。 (2)HVFEF treatment：Under the conditions of 4kHz木4kWcm木6min，the mutation rate Was the highest，shows(1 7．96士1．43)水1 0～，were 1 4．97 times of spontaneous mutation rate(1．20’Ic 10。6)and 7．16 times ofdrying control group(2．51 水1 0‘6)．The result