多不饱和脂肪酸对人脐带间充质干细胞生物学特性的影响生物工程专业论文.docxVIP

多不饱和脂肪酸对人脐带间充质干细胞生物学特性的影响hUC．MSCs增殖、迁移及神经分化。本实验的研究成果有望用于细胞替代治疗中， 多不饱和脂肪酸对人脐带间充质干细胞生物学特性的影响 hUC．MSCs增殖、迁移及神经分化。本实验的研究成果有望用于细胞替代治疗中， 多不饱和脂肪酸增补培养hUC．MSCs后，分化成可行使功能的神经细胞，替代引 起病变的已坏死的神经细胞，以达到减缓或治愈神经退行性疾病的目的。 关键词：人脐带间充质干细胞；多不饱和脂肪酸；神经元细胞；神经分化 万方数据 内蒙古大学硕士学位论文EFFECTS 内蒙古大学硕士学位论文 EFFECTS OF PO LyUNSATURATED R气TTY ACIDS ON THE B10LOGICAL CHARACTERISTICS OF HUMAN UMBILICAL CORD MESENCHYMAL STEM CELLS AB STRACT Mesenchymal stem cells are one of the important members of adult stem cell s， having pluripotency and self-renew ability．In 2002，Woodbury et a1．demonstrated for the first time that MSCs could differentiate into neurons in vitro．At present，there are four methods to induce MSCs differentiate into neurons in vitro，which are anti oxidant，cytokine，Chinese herbal medicine and gene editing．The obj ective of our study was to make the MSCs derived neuron like cells have more characteristics of normal neurons． Omega-3 and omega-6 families of polyunsaturated fatty acid(PUFA)and its metabolic products have effects on cell structure and function，they can promote stem cell proliferation and differentiation，then affect the fate of stem cells．In our study， human umbilical cord mesenchymal stem cells(hUC．MSCs)were cultured with PUFA supplement，including eicosapentaenoic acid(EPA)and docosapentenoic acid 万方数据 (DPA)．MTS (DPA)．MTS Assay and Transwell migration experiment showed that EPA and DPA could promote the proliferation and migration of hUC·MSCs．The expression of neuronal specific markers in hUC-MSCs differentiated neurons after addition of EPA and DPA was higher than that in the control group，including glial fibrillary acidic protein(GFAP)，neurofilament(NF)，tyrosine hydroxylase(TH)，neuron specific nuclear antigen(NeuN)，neuron specific dilute enolase background enolase (NES)and microtubule associated protein 2(MAP2)．These results showed that EPA and DPA are involved in the proliferation，migration and differentiation of hUC．MSCs．The results are expected to be used in the treatment o