家兔遗传多样性及系统发生关系微卫星分析.pdfVIP

生物多样性 2008, 16 (5): 492–497 doi: 10.3724/SP.J.1003.2008.08051 Biodiversity Science http: // 家兔遗传多样性及系统发生关系的微卫星分析 * 孙红梅 邢秀梅 荣 敏 丛 波 (中国农业科学院特产研究所, 吉林左家 132109) 摘要: 为了揭示我国家兔资源的遗传现状, 本文从30个微卫星位点中筛选出10个位点, 对22个家兔品种(系) 的遗 传变异进行了分析。结果表明: 22个家兔品种(系)在10个微卫星座位上的平均有效等位基因数为3.0–4.6; 平均多态 信息含量在0.4997–0.7009之间, 多为高度多态, 说明家兔有丰富的遗传多样性。各品种之间的遗传距离(DS)较近, 在0.0087–1.2712之间, 其中以美系獭兔和德系獭兔间距离最近, 为0.0087 。德国大白兔与长毛兔之间距离最远, 为 1.2712。根据遗传距离做聚类图, 可以看出中国22个家兔品种被分为三大类: 吉戎- Ⅰ系和吉戎- Ⅱ系成一类, 塞北 兔、哈白兔聚为一类, 其他品种聚为一大类。该研究结果将为中国家兔品种的保护和利用提供重要的科学依据。 关键词: 家兔, 微卫星标记, 遗传多样性, 系统发生关系 Genetic diversity and phylogenetic relationship of rabbit breeds based on microsatellite DNA markers * Hongmei Sun , Xiumei Xing, Min Rong, Bo Cong Institute of Special Economic Plants and Animals, Chinese Academy of Agricultural Sciences, Zuojia, Jilin 132109 Abstract: Genetic diversity of 22 rabbit breeds were investigated using 10 microsatellite DNA markers. The results showed that the average number of effective alleles ranged from 3.0 to 4.6, and the polymorphism in- formation content (PIC) ranged from 0.4997 to 0.7009, showing highly polymorphic. The lowest genetic distance (0.0087) was observed between American rex rabbit and Germany rex rabbit, while the highest ge- netic distance (1.2712) was observed between Germany white rabbit and Changm. UPGMA cluster analysis based on genetic distance showed that the 22 breeds could be divided into three groups. The first group con- tained Jirong- Ⅰand Jirong- Ⅱ rabbit, Saibei rabbit and Habai rabbit comprised a second group, and the other