鲍曼不动杆菌耐药程度与其主动外排泵蛋白的相关性研究医学微生物学专业论文.docxVIP

硕士学位论文 硕士学位论文 中文摘要 摘要 目的了解长沙地区鲍曼不动杆菌的耐药现状和耐药水平，为临 床治疗鲍曼不动杆菌感染、合理应用抗生素提供实验依据和指导；研 究鲍曼不动杆菌耐药程度与其主动外排泵蛋白的相关性，初步探讨鲍 曼不动杆菌主动外排泵基因过度表达的转录调控机制。 方法首先用纸片扩散法检测64株临床鲍曼不动杆菌对8种抗菌 药物的敏感性；将其分为A组(对1～2种抗生素耐药)、B组(对3～ 5种抗生素耐药)和C组(对6,--．,8种抗生素耐药)；检测64株临床 鲍曼不动杆菌对罗丹明6G的外排情况，筛选出罗丹明6G外排明显增 加的菌株；并用逆转录一聚合酶链反应(RT．PCR)方法检测主动外排 泵基因adeABC的表达水平；应用PCR方法扩增adeRS基因片段并 测序，探索adeABC基因表达上调的可能机制。 结果64株鲍曼不动杆菌中有4株对1～2种抗生素耐药(A组)， 对3--5种抗生素耐药的有33株(B组)，对6～8种抗生素耐药的 有27株(C组)； 多重耐药组鲍曼不动杆菌对罗丹明6 G外排明显 增高，外排程度A组B组C组；罗丹明6G外排增强的多重耐药不 动杆菌其主动外排泵基因adeABC的表达水平显著增高；且存在AdeR Val228一Ile、Ile243一Val变异。 硕士学位论文 硕士学位论文 中文摘要 结论①长沙地区鲍曼不动杆菌的耐药现象严峻；②鲍曼不动杆 菌多重耐药与药物主动外排增强有关；@adeABC基因的过度表达是 鲍曼不动杆菌多重耐药的重要原因。④Adel宅S中的AdeR Val228---，Ile、 Ile243一Val变异与adeABC表达上调相关。 关键词 鲍曼不动杆菌；主动外排泵；多重耐药；adeABC 硕士学位论文 硕士学位论文 英文摘要 Abstract obj ective To understand the A cinetobacter baumannii strains present situation and levels of the drug resistance in Changsha，to provide the basis for the reasonable clinical application of the antibiotics；To investigate the function and gene expression of active efflux transporters in Acinetobacter baumannii，and initial exploration of the mechanisms of transcription regulation of the active efflux transporters． Methods At first，the susceptibilities of 64 strains clinical Acinetobacter baumannii to 8 kinds of antibiotic agents were tested by disc diffusion method，then these strains were divided into three groups based on the susceptibilities：group A(resistant to 1--2 antibiotics)， group B(resistant to 3～5 antibiotic)and group C(resistant to 6～8 antibiotics)；The efflux of Rhodamine 6G in these 64 strains of clinical A cinetobacter baumannii was tested and the strains with high increased efflux of Rhodamine 6G were identified； Reverse transcriptase polymerase chain reaction(RT—PCR)was used to measure the mRNA expression levels of active efflux gene adeABC；To explore the mechanisms of overexpression of adeABC，the fragments in adeRS were amplified by PCR and directly sequenced． Results Among the 64 strains，4 were resistant t