高糖对肾小球系膜细胞生物钟基因表达的影响及机制探讨内科学专业论文.docxVIP

西南医科大学硕士学位论文CLOCK，BMALl，REV—ERBa,Perl，Per2，Per3，C巧1，C巧2 西南医科大学硕士学位论文 CLOCK，BMALl，REV—ERBa,Perl，Per2，Per3，C巧1，C巧2 mRNA 表达水平均增加(PO．05)，其中CLOCK，BMALl，REV．ERBa， Perl，Per2，Per3，C巧2 mRNA表达增加呈浓度依赖性；同时，与正 常对照组相比，高糖组小鼠肾小球系膜细胞内ROS水平及细胞培养 上清液中IL一6及CCL．2表达水平均呈浓度依赖性增加(PO．05)。2． 与高糖组相比，预先加入NAC干预高糖氧化应激作用后可阻止高糖 诱导的小鼠肾小球系膜细胞CLOCK，BMALl，REV．ERBa，Perl， Per2，Per3，C巧1，CU2 mRNA高表达(PO．05)，加入NAC干预 高糖作用后同样能降低小鼠肾小球系膜细胞内ROS水平及细胞培养 上清液中IL．6、CCL．2表达水平(PO．05)。结论：高糖通过氧化应 激机制上调小鼠肾小球系膜细胞生物钟基因CLOCK，BMALl， REV—ERBa，Perl，Per2，Per3，CWl，C拶2的表达，可能参与了糖尿 病肾病炎症反应的发生。 关键词：糖尿病；生物钟；氧化应激；炎性介质。 万方数据 西南医科大学硕士学位论文The 西南医科大学硕士学位论文 The effect of high glucose on the expression of circadian clock gene in glomerular mesanginai cell Abstract Objective：Circadian rhythm is one of the most popular phenomenon of the nature，circadian clock is the system which set and regulate this kind of day and night cycle of the body,and is a kind of the circadian oscillator for a period which is approximately 24 hours． Circadian clock is synchronize with sunlight cycle，metabolism and energy intake．A number of studies have found that the circadian clock gene expression is closely related to blood glUcose level，circadian rhythm disorder is one of the risk factors of diabetes mellitus(DM)，but there i s no research to study the effect of high glucose on the circadian clock gene expression before，SO this research observes the expression of circadian clock genes stimulated by different concentrations of high glucose and reactive oxygen species(ROS)inhibitor which is named N—acctyl-L-cystcinc(NAC)in mouse glomerular mesanginal cells，we detect the ROS level and expression of inflammation factors at the same time，aiming to explore the control effects and corresponding mechanism of the high glucose in circadian clock expression and inflammatory reaction．Methods：1．Mouse glomerular mesangial cells cultured with 万方数据 西南医科大学硕士学位论文different 西南医科大学硕士学位论文 different concentrations of high glucose as stimulating factors，NAC as the intervention fa