餐厨垃圾堆肥过程中水解酶活性变化的研究-中国农学通报.PDFVIP

中国农学通报 2012,28(11):284-288 Chinese Agricultural Science Bulletin 餐厨垃圾堆肥过程中水解酶活性变化的研究 牛俊玲，郑宾国，梁丽珍 （郑州航空工业管理学院资源与环境研究所，郑州450015 ） 摘 要：为了研究餐厨垃圾在静态强制通风条件下堆腐过程的矿质化进度和强度，以小麦秸秆作为膨松 剂，比较了不同C/N 比(20 ，25 ，30)条件下餐厨垃圾堆肥过程中各个阶段纤维素酶、蔗糖酶、脲酶、蛋白酶 4 种水解酶活性的变化情况。结果表明：不同C/N 比处理的温度变化趋势基本相同，40℃以上高温期持 续达10 天左右。25C/N 和30C/N 堆肥条件下的纤维素酶活整体比20C/N 要高，酶活高峰期出现滞后于温度高 峰期；不同C/N 条件下，蔗糖酶活性变化趋势基本相似，酶活高峰期与温度高峰期同步；30C/N 条件下的脲 酶活性峰值出现早于20C/N 和25C/N ，最终脲酶活性下降到比堆肥初期还要低的程度；高温对蛋白酶活性有 抑制作用，25C/N 条件下蛋白酶活性峰值出现晚于其他2 个处理。从试验结果可以看出，不同营养条件下 同类酶活性的变化趋势表现出一定的差异，与堆肥过程中有机物质的转化密切相关，可以作为判断堆肥 腐熟度的因素之一。 关键词：餐厨垃圾；堆肥；碳氮比；水解酶 中图分类号：X7 文献标志码：A 论文编号：2011-3291 StudyontheChangeofHydrolyticEnzymeActivityDuringtheCompostingProcessofKitchenWastes Niu Junling, Zheng Binguo, Liang Lizhen （InstituteofResourcesandEnvironmentalScience, ZhengzhouInstituteofAeronauticalIndustryManagement, Zhengzhou 450015) Abstract: In order to study the mineralization process of organic substances, the composting for kitchen wasting mixed with wheat straw was carried out by static forced ventilation and under the conditions of different C/N ratio as 20, 25, 30, and the activities of Cellulase, Sucrase, Urease and protein enzyme were tested respectively at the different stages of composting. The results showed that the variation trends of temperature was similar for C/N ratio conditions, the temperatures above 40℃ lasted for about 10 days. The Cellulase activities under the conditions of 25C/N and 30C/N were higher than those of 20C/N, and the peaks of enzyme activity were lagged behind of the temperature peaks. Under different C/N conditions, the variation tre