膀胱移行细胞癌中淋巴管密度与血管内皮生长因子d表达的研究泌尿外科专业论文.docxVIP

优秀毕业论文 精品参考文献资料 硕士学位论文 硕士学位论文 中文摘要 摘 要 目的： 1)探讨淋巴管生长因子VEGF．D在膀胱移行细胞癌(BTCC)中 的表达及其与临床相关因素的关系； 2)探讨膀胱移行细胞癌淋巴管密度与临床相关因素的关系； 3)探讨肿瘤VEGF．D表达与淋巴管密度的相关性。 方法： 1)采用免疫组织化学方法研究50例膀胱移行细胞癌中VEGF．D 蛋白表达的水平，评估VEGF．D表达与临床病理资料的相关性； 2)免疫组化方法直观显示肿瘤淋巴管形态结构，并计数淋巴管密 度，研究淋巴管密度与临床病理指标的关系； 3)分析肿瘤VEGF．D表达与淋巴管密度的相关性。 结果： 1)50例中ⅦGF．D阳性42例；VEGF．DISH性表达与肿瘤分级、分期 及大小明显相关(PO．05)；与复发性无关(PO．05)； 2)肿瘤LVD明显高于正常组织(PO．001)；LVD与肿瘤分级相关 (P0．05)，与分期、大小及复发性无关@O．05)； 3)VEGF．D表达与淋巴管密度显著相关(PO．05)。 结论： 1．在BTCC，VEGF．D表达水平明显增强且与病理分级、临床分期及 肿瘤大小呈正相关，可能作为诊断和判定预后的一个指标。 2．LVD与BTCC的病理分级呈正相关，可能作为判定肿瘤细胞恶性程 度的指标。 3．VEGF—D的表达与BTCC中LVD呈正相关，VEGF—D表达可能与淋巴 管生成有关，VEGF．D可能成为一项新的治疗靶点。 关键词 膀胱移行细胞癌，VEGF．D，淋巴管密度， 预后 硕士学位论文 硕士学位论文 英文摘要 Research on lymphatic vessel Density and the Expression of Endothelial Vascular Growth Factor．D in bladder transitiOnal cell Carcinoma ABSTRACT oBJECTIVE： 1)To investigate expression and clinical significance of VEGF—D in Bladder Transitional Cell Carcinom． 2)To determine tumoral lymphatic vessel density(LVD)in BTCC and its correlation wi出clinical findings． 3)To evaluate the relationship of the expression of VEGF—D and LⅧ． METHoD： 1)We assessed the expression of VEGF—D by immunohistochemistry in 50 Bladder Transitional Cell Carcinomas．The relationship VEGF—D expression and established clinicopathological parameters was assessed． 2)Lymphatic vessels was immunostained with D2-40 and LVD evaluated in tumoral areas．We assessed the relationship LVD and established clinicopathological parameters． 3)To analyze the relationship of the expression of VEGF—D and LVD． RESULTS： 1)VEGF-D expression was identified in 42 cases(84％)with in immunohistochemistry．Higher expression of VEGF—D was significantly correlated with poor histologic grade，clinical stage and tumor size (PO．05)． 2)The LVD was significantly higher in stromal tissue of tumors than in that of normal tissues(P0．00 1)．LVD was significantly higher in II high-grade high-grade tumors than that in low-grade tumors(P0．05)．LVD was not associated with pT stage