不同诱导子对酿酒葡萄赤霞珠组培苗白藜芦醇含量及抗氧化酶活性的影响园艺专业论文.docxVIP

摘要本研究以多年生酿酒葡萄赤霞珠的幼嫩茎段为外植体，并研究其组培苗的最适培养条 摘要 本研究以多年生酿酒葡萄赤霞珠的幼嫩茎段为外植体，并研究其组培苗的最适培养条 件；以培养出的组培苗为材料，通过添加灰葡萄孢诱导子和水杨酸，研究诱导子对赤霞珠葡 萄组培苗中白藜芦醇合成及抗氧化酶活性的影响。主要研究结果如下： 1．以赤霞珠葡萄新梢的半木质化茎段作为外植体材料，接种培养污染率低，外植体萌 芽率高，萌芽率为96．5％；消毒溶液以0．1％升汞溶液消毒8min效果最好。继代培养和生根 培养以1／2MS+IBA0．2mg·L‘1．-I-蔗糖209·L-1培养基培养赤霞珠葡萄组培苗生长状况最好，成苗 率为99．67％，生根率为99．67％。 2．灰葡萄孢诱导子和水杨酸诱导赤霞珠组培苗，均是诱导9d的效果最好，其中，灰葡 萄孢诱导子可以使白藜芦醇含量比对照高近1．67倍；水杨酸可以使白藜芦醇含量比对照高 1．5倍；因此，灰葡萄孢诱导子对赤霞珠组培苗白藜芦醇的促进效果比水杨酸好。 3．灰葡萄孢诱导子处理后赤霞珠组培苗中PAL、POD、SOD、CAT、PPO活性均大于 对照，分别是对照的2．17、1．58、1．48、1．83、2．11倍。水杨酸处理后赤霞珠组培苗中PAL、 POD、SOD、CAT、PPO活性均大于对照，分别是对照的l，37、1．13、1．23、I．03、2．19倍。 4．在诱导第6．9d时，白藜芦醇含量增加，抗氧化酶及苯丙氨酸解氨酶、多酚氧化酶活 性均增加。 关键词：赤霞珠，组培苗，白藜芦醇，抗氧化酶，灰葡萄孢，水杨酸 万方数据 AbstractThis Abstract This study with tender stems of perennial grapevine Cabernet Sauvignon explants，in the cultured plantlets materials，by adding Botrytis cinerea elicitor and salicylic acid，resveratrol synthesis and antioxidant enzyme activity process of Cabernet Sauvignon grape tissue culture seedlings elicitor the effect．The main results follows： 1．The semi woody Cabernet Sauvignon grape shoots of stem segment explant material， low contamination rate of explants inoculated with hi。gh germination rate，germination rate was 96．5％；in 0．1％HgCl2 solution disinfection disinfection solution 8min had the best effect．． Subculture and rooting culture of Cabernet Sauvignon seedlings was the best medium with 1／2MS+IBA0．2mg’L。1+sucrose 209‘L～，the seedling rate was 99．67％，the rooting rate was 99．67％． 2．The result showed that Botrytis cinerea elicitor and SA induced Cabernet Sauvignon tissue culture seedlings were induced by 9d effect is the best．And Botrytis cinerea elicitor treatment make the content of resveratrol in nearly 1．67 times higher than that of the contr01．SA treatment make the content of resveratrol in nearly 1．5 times higher than that of the contr01．Therefore， the effect of resveratrol Botrytis cinerea was beRer than SA． 3．Botrytis cinerea elicitor treatment PAL，POD，SOD，CAT，PPO activity of Cabernet Sau