井冈山产凹叶厚朴挥发油中化学成分分析.PDFVIP

中草药 =q==e=a 第 QS 卷 第 OQ 期 =OMNR 年 NO 月 ·PSQV · 井冈山产凹叶厚朴挥发油中化学成分分析 曾 红，邓先清，黄玉珊 井冈山大学医学院，江西 吉安 343000 摘 要：目的 对井冈山产凹叶厚朴 Magnolia officinalis var. biloba 果实、花、干皮、叶 4 个部位的挥发油成分进行分析，为 厚朴不同部位的入药性质提供参考。方法 采用水蒸气蒸馏法提取厚朴 4 个部位的挥发油，运用气相色谱-质谱联用（GC-MS ） 技术对挥发油成分进行分析和鉴定，采用峰面积归一化法确定各成分的质量分数。结果 从井冈山产凹叶厚朴的挥发油中共 鉴定出 75 个化学成分，其中果实中鉴定出 1-石竹烯、α-蒎烯等 42 个成分，质量分数占 98.7%；花中鉴定出 4-羟基-4- 甲基-2- 戊酮、茨烯等 19 个成分，质量分数占 77.59% ；干皮中鉴定出α-桉叶油醇、四十四烷等 24 个成分，质量分数占 77.92% ；叶 中鉴定出 1-石竹烯、α-桉叶油醇等 32 个成分，质量分数占 76.48% 。1-石竹烯和四十三烷为 4 个部位共有。结论 从凹叶 厚朴挥发油成分角度，凹叶厚朴叶可替代厚朴皮入药，果实可以入药，花活性成分量很少，不建议入药。 关键词：厚朴；凹叶厚朴；挥发油；气相色谱-质谱联用；1-石竹烯 中图分类号：R284.1 文献标志码：A 文章编号：0253 - 2670(2015)24 - 3649 - 06 alfW 10.7501/j.issn.0253-2670.2015.24.006 K g ZENG Hong, DENG Xian-qing, HUANG Yu-shan College of Pharmacy, Jinggangshan University, Ji’an 343000, China W l To research the constituents of the volatile oil from the fruits, flowers, skins, and leaves of Magnolia officinalis var. biloba produced in Jinggangshan. This research will provide the medicinal nature proof for the different parts of M. officinalis var. biloba. j The volatile oil from M. officinalis var. biloba was extracted by steam distillation, and the components of the volatile oil were separated and structurally identified by gas chromatlgraphy-mass spectrometry. The relative percentage of various volatile oil ingredients was determined by the area normalizing method in gas chromatography. o All 75 compounds were identified from M. officinalis var. biloba in Jinggangshan. Among them 42 compounds like 1-caryophyllene and α-pinene in nuts were identified and the relative content was 98.7%. Nineteen compounds like 4-hydroxy-4-methyl-2-pentanone and camphene in the flowers were identified and the relative content was 77.59%. Twenty-four compounds like α-eudesmol, and 44 alkanes in the skins wer